We narrowed to 740 results for: NSI

... a number of different options. By carefully considering your ncRNA of interest, you should be able to...

...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

...activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity to be ...

... starting point for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally...

...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...

...Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang Ligation Fidelity...

...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

...of toehold switch-based RNA sensors within an extensive infrastructure of molecular and electronic companion...

...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...

...inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts...

...it’s too inefficient and the reagents are too expensive to make it practical - but we have members looking...

...deposition of the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into...

.... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...

...cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final...

...own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a...

...think plant biology is massively underfunded considering how important it is. I would really like to reach...

...multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial genomes with...

...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...

...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...

...streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display...

...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

...the labeled regions, as well as the strength and density of labeling in each. This allows users to quickly...

...plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free genome editing...

...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...

...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...

...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

... and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes...

.... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture...

...unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...

...different universities are trying to compile more comprehensive demographic information about the postdoc work...

...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

...cells. For example, KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced...

...transformants for appropriately sized inserts. We consider a library worth using if at least 40% of colonies...

...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the...

... scientific community and  remind us to always consider the ethics and broader implications of our research...

...be expressed simultaneously. Vectors for both transient expression and Agrobacterium-mediated transformation...