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Showing: 281 - 300 of 545 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...bricks” to be added one by one. This approach is especially useful for combinatorial analyses. For example... L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an aTet-inducible gRNA and...

  2. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed in a 1:1 ratio and...the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... to de-differentiate. However it’s difficult, especially as a newbie reprogrammer, to decide which method...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors...

  5. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019; Trampuz...you’re using a different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...primary antibodies can be more difficult to source, especially if you need a specific conjugate, and changing...detection quickly (~1 minute), and have a linear response over a broad dynamic range that typically spans...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... protein increases (Figure 3A).  This step is especially important for highly expressed proteins, including... with biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this...

  8. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ... what antibodies are, their role in the immune response, and the different antibody isotypes found in ...

  9. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...underrated and I recommend checking them out, especially since videos on synthetic biology can be difficult...

  15. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...-intensive systems in a more environmentally responsible manner.  Other ways to make the office more eco-friendly...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...able to access these snippets here or from their respective articles on the Hot Plasmid webpage. We hope ...

  20. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...of lab materials to create their decorations! Despite all the craziness of 2020, for our Zoom #HolidayParty...
Showing: 281 - 300 of 545 results