We narrowed to 559 results for: ESP

...validation techniques for academia, industry, and especially the clinic. Popular validation assays are insufficient...search the whole genome for off-target sites irrespective of in silico prediction. These techniques differ...

...lower than the energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher ...FRET).   Figure 1: Emission spectra and respective colors of a few commonly used fluorophores. Each...

...sequences at the intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid ...researchers from generating clonal knock-in lines, especially for ambitious projects that require epitope tagging...

...through the excitation light path. Each hole corresponds to a different location in the sample. As more...step that reassigns out of focus light to its respective focal plane. In sufficiently thin samples, deconvolution...

... of a pathway to inhibit to block the cellular response to a particular ligand (such as when a component...pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

...mastered this technique so it might be worth a try, especially since the potential rewards could be substantial...) distance at which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET...

...bricks” to be added one by one. This approach is especially useful for combinatorial analyses. For example... L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an aTet-inducible gRNA and...

...labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed in a 1:1 ratio and...the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies...

...caused by not having the time to assess public response; and, it is the stage I think we are currently...effort from the community in order to succeed. With respect to de novo sgRNA design, I again separate it into...

... peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019; Trampuz...you’re using a different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as...

... to de-differentiate. However it’s difficult, especially as a newbie reprogrammer, to decide which method...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors...

...primary antibodies can be more difficult to source, especially if you need a specific conjugate, and changing...detection quickly (~1 minute), and have a linear response over a broad dynamic range that typically spans...

... protein increases (Figure 3A).  This step is especially important for highly expressed proteins, including... with biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this...

... what antibodies are, their role in the immune response, and the different antibody isotypes found in ...

...resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...

... details that will help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet...

...over, but signs of holiday spirit still linger (despite the unseasonably warm weather we had last weekend...

...underrated and I recommend checking them out, especially since videos on synthetic biology can be difficult...

...” into the GIF search bar to find that perfect response GIF.     We have GIFs for every occasion...

...plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike many commercially available ladders,...