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We narrowed to 554 results for: ESP

Showing: 281 - 300 of 554 results

  1. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...through the excitation light path. Each hole corresponds to a different location in the sample. As more...step that reassigns out of focus light to its respective focal plane. In sufficiently thin samples, deconvolution...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... of a pathway to inhibit to block the cellular response to a particular ligand (such as when a component...pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...lower than the energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher ...FRET).   Figure 1: Emission spectra and respective colors of a few commonly used fluorophores. Each...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...mastered this technique so it might be worth a try, especially since the potential rewards could be substantial...) distance at which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...bricks” to be added one by one. This approach is especially useful for combinatorial analyses. For example... L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an aTet-inducible gRNA and...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed in a 1:1 ratio and...the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... to de-differentiate. However it’s difficult, especially as a newbie reprogrammer, to decide which method...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019; Trampuz...you’re using a different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...primary antibodies can be more difficult to source, especially if you need a specific conjugate, and changing...detection quickly (~1 minute), and have a linear response over a broad dynamic range that typically spans...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... protein increases (Figure 3A).  This step is especially important for highly expressed proteins, including... with biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this...

  12. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ... what antibodies are, their role in the immune response, and the different antibody isotypes found in ...

  13. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...

  16. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...underrated and I recommend checking them out, especially since videos on synthetic biology can be difficult...

  19. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...-intensive systems in a more environmentally responsible manner.  Other ways to make the office more eco-friendly...
Showing: 281 - 300 of 554 results