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We narrowed to 566 results for: FIE

Showing: 281 - 300 of 566 results

  1. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, ...your favorite model system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without a purpose...Backbone: The starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector...

  3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ... protein, RNA, or DNA. Finally, once you have identified the best expression system, you can then choose...embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on ... alcohol and methyl salicylate which was later modified by others to produce Murray’s clear solution.  ...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission...

  7. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...you than your technical expertise in a specific field. While searching for jobs and making connections...identify it as a personal strength until it is identified by another person. Similar reasoning may also...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... termini. It doesn’t matter if the DNA is PCR amplified, restriction digested or even a synthesized gene... are assembled in vivo to produce the multiply modified product. For example, by combining multiple primers...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different problem...out tables 1 and 2 below for a summary of the quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S...

  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...and off-target efficiency When Cpf1 was first identified, we didn't know much about its on-target and ...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Secondly, 3rd generation packaging systems have modified the 5’ LTR of the transfer plasmid to eliminate...cellular genes. Q7: Which genes are deleted or modified to result in replication deficiency? What percent...

  14. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...method with Agrobacterium: If you are new to the field, the following are useful tips for your first transient... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

  15. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...mouse (Zhou et al., 2018). This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits...

  16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding... be used to modify genomic loci that cannot be modified using wild-type SpCas9. The number of off-target...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...sequence-specific protease used to cleave affinity tags from purified recombinant proteins at small and large scale....biotinylation. Once induced, the NBs could be easily purified by one-step immobilized metal ion adsorption chromatography...

  20. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...and other online career resources Once you’ve identified places you’d like to work, get online and take... a personal connection. That way, once you’ve identified an appropriate job opening, you can send your...
Showing: 281 - 300 of 566 results