We narrowed to 354 results for: HRE

1. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...should ponder this figure often, especially the three steps from the 12 o’clock to the 6 o’clock positions...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...part system (figure 4d):  Part 1: A cocktail of three AAV vectors expressing RGB proteins under the control...

3. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ...about how to balance those roles. I’m a father of three, a scientist and science communicator, and I strive...

4. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ... will focus the rest of our discussion on these three regions. Each of these regions was affected at a...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular expression...

6. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...inactive Cas13) to target the cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues...

7. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...data was published.    Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into...

8. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and transfer ...

9. Plasmids 101: Colony PCR

   Type

   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ...summarizes generalized expected results for the three primers previously described. When using insert-...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...Fridays. From each overnight culture, we prepare three sets of 2D barcoded tubes. Two of the tubes are ...

11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...tendency is one of the reasons why we have only three biology-related Nobel prizes, out of which only ...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...chromophore formed from a maturation reaction of three specific amino acids at the center of the protein...

13. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...collaborators’ websites, or as a recommendation in a Reddit thread crowdsourcing experimental help. We love it when...

14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ... and jGCaMP8 per year from 2016–2023. All three generations are regularly requested and a valuable...

15. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... The body of the cover letter should be one to three paragraphs. This is where you make the case for ...

16. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...cells and dendritic cells is further separated into three different populations using conjugated antibodies...

17. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...sections of tissue, the Wu Lab is working to create a three-dimensional atlas of protein expression in the adult...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue), which...

19. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ... 6-well dish and change the media every day for three days. GVs should be visible under phase contrast...