We narrowed to 316 results for: Mut
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TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids...
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Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation consensus... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m.... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m.... fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings of the... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m.... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....process makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially...