We narrowed to 362 results for: Numb

1. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...titer your virus. Getting an accurate count of the number of infectious particles in a given viral stock ...

2. Plasmids 101: FLEx Vectors

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   Blog Post

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   April 28, 2016, 2:30 p.m.

   ...will excise the DNA fragment. By manipulating the number, orientation, and type of target sites that flox...

3. The Materials Science of Optogenetics Experiments

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   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...during the course of an experiment. There are a number of reasons for this but the primary one is that...

4. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

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   July 23, 2015, 2:30 p.m.

   ... good ways to monitor and track them. We have a number of microscopes, including an automated microscope...

5. Pooled Library Amplifications

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   Blog Post

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   May 30, 2024, 1:30 p.m.

   ...Libraries at Addgene.  Library Type Name Number of Plasmids Variable Region Size DNA per Amplification...

6. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

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   Oct. 12, 2017, 2:26 p.m.

   ...the addition of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers...

7. Degrading DNA with Cascade-Cas3

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   Blog Post

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   Feb. 11, 2025, 2:15 p.m.

   ...versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base...

8. Viral Vectors 101: Voltage Indicators

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   Blog Post

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   Jan. 30, 2024, 2:15 p.m.

   ...specific example is attractive due to the large number of sheer molecules and signal you can generate ...

9. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

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   Aug. 3, 2023, 1:15 p.m.

   ...the better choice. Of note, there is no minimum number of markers that needs to be used with spectral ...

10. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

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    Sept. 7, 2017, 12:17 p.m.

    ...advantage of this technique is the potentially large number of single clones obtained (on occasion we have ...

11. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

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    Sept. 6, 2016, 2:30 p.m.

    ...Nashville, Tennessee has already demonstrated that a number of novel small molecules can bind directly, albeit...

12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

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    April 19, 2016, 2:30 p.m.

    ...things are not quite so simple. Concentration is the number of molecules within a volume. In many biological...

13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...about step 3, however, you may be surprised at the number of options available - how do you choose between...

14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

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    May 22, 2015, 4:58 p.m.

    ...risk a potential way to dramatically reduce that number for the sake of laboratory convenience. Disclaimer...

15. Plasmids 101: Multicistronic Vectors

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    Blog Post

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    Sept. 9, 2014, 8:20 p.m.

    ...specific 2A peptide will ultimately depend on a number of factors such as cell type or experimental conditions...

16. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post

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    Aug. 13, 2024, 1:15 p.m.

    ...bands or a few strong ones, consider increasing the number of washes performed during your harvest. Validation...

17. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

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    Feb. 27, 2018, 2:04 p.m.

    .../or a 3x cysteine varieties. This refers to the number of cysteine sites the nanobody has available for...

18. Getting to Know Expansion Microscopy

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    Blog Post

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    June 20, 2023, 1:15 p.m.

    ...molecular biology lab, and EM is limited in the number of specific targets you can label in a sample. ...

19. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ... and the lines’ genomes, and a reduction in the number of the dependencies detected. Given this new information...

20. AAVs in Retinal Gene Therapy

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    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...successes of retinal gene therapy, an ever-growing number of clinical trials for devastating genetic diseases...