We narrowed to 370 results for: Numb

1. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways can require...

2. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...from troponin C. It is engineered with a reduced number of calcium binding sites per sensor (to decrease...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ... entirely remains a large undertaking. Priority number one was to ensure that this changeover had no impact...

4. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...amplification primers. This helped make sure that we had a number of successful sensors to draw on as our assays ...

5. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...titer your virus. Getting an accurate count of the number of infectious particles in a given viral stock ...

6. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...will excise the DNA fragment. By manipulating the number, orientation, and type of target sites that flox...

7. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...during the course of an experiment. There are a number of reasons for this but the primary one is that...

8. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ... good ways to monitor and track them. We have a number of microscopes, including an automated microscope...

9. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   Published

   Feb. 11, 2025, 2:15 p.m.

   ...versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base...

10. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...Libraries at Addgene.  Library Type Name Number of Plasmids Variable Region Size DNA per Amplification...

11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...the addition of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers...

12. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...advantage of this technique is the potentially large number of single clones obtained (on occasion we have ...

13. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...specific example is attractive due to the large number of sheer molecules and signal you can generate ...

14. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...the better choice. Of note, there is no minimum number of markers that needs to be used with spectral ...

15. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...Nashville, Tennessee has already demonstrated that a number of novel small molecules can bind directly, albeit...

16. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...things are not quite so simple. Concentration is the number of molecules within a volume. In many biological...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...about step 3, however, you may be surprised at the number of options available - how do you choose between...

18. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...risk a potential way to dramatically reduce that number for the sake of laboratory convenience. Disclaimer...

19. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...specific 2A peptide will ultimately depend on a number of factors such as cell type or experimental conditions...

20. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...successes of retinal gene therapy, an ever-growing number of clinical trials for devastating genetic diseases...