We narrowed to 553 results for: PPL
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  TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...
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  When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in CellsTypeBlog PostPublishedApril 19, 2016, 2:30 p.m....problem for fusion proteins. One of the major applications of FPs is to visualize the localization, dynamics...An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein...
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  Transgenic Organisms, Cas9 Gene Drives, and Appropriate SafeguardsTypeBlog PostPublishedMay 22, 2015, 4:58 p.m....species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue ...
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  INTEGRATE: Bacterial Genome Engineering Using CRISPR-TransposonsTypeBlog PostPublishedDec. 7, 2020, 2:53 p.m.... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated...
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  Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions EasyTypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....PIPs, resulting in a fluorescent readout. Applications for bimolecular fluorescence complementation...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because...
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  Capturing a Moment with Marc Zimmer’s “The State of Science”TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m.... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...successful scientists have outgoing personalities to supplement their intellectual prowess and scientific skills...
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  The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene DataTypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical methods for fundamental...fundamental scientific discovery and high-impact applications. Alvin Hsu is a graduate student at Harvard...
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  An Introduction to AdenovirusTypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....Persistent infection by adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136...on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting...
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  Getting to Know Expansion MicroscopyTypeBlog PostPublishedJune 20, 2023, 1:15 p.m....with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...
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  Great Results Start with Great Standard CurvesTypeBlog PostPublishedAug. 15, 2024, 1:15 p.m.... critical for a number of popular scientific applications, and the quality of a standard curve can make...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95. Additional...
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  A Guide to Starting Your Own Journal ClubTypeBlog PostPublishedFeb. 6, 2020, 3 p.m....notice typically should include the paper, any supplemental data, software repositories, or other information.... These variations are suited for different applications and should be carefully chosen for each group...
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  CRISPR AntimicrobialsTypeBlog PostPublishedMay 3, 2016, 2:30 p.m.... to eliminate the use of antibiotics by their suppliers, and public health and medical organizations are...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...
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  Cpf1: A New Tool for CRISPR Genome EditingTypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....-target editing in a cell culture model. The application of Cpf1 to genome editing is exciting both in...in terms of basic science and translational applications. This discovery of this type V CRISPR system ...
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  Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and MoreTypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'...
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  Your Lentiviral Plasmid FAQs AnsweredTypeBlog PostPublishedApril 23, 2014, 1:08 p.m....lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated knockdown...type or tissues; this is useful in gene therapy applications where a distinct subset of the cellular population...
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  Designing Your Chalk Talk for the Academic Job InterviewTypeBlog PostPublishedMarch 12, 2019, 1:37 p.m..... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet...of common questions, check out this eBook about applying for faculty positions. You need to practice with...
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  Lab to Office Culture ShockTypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m.... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...thinking” in the position to which she was applying. I probably did not respond to her question as well then...
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  Rett Syndrome: A History of Research and Therapeutic OutlooksTypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon...
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  Overcoming the AAV Size Limitation for CRISPR DeliveryTypeBlog PostPublishedSept. 16, 2020, 1 p.m....finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...https://doi.org/10.1126/science.aaq0180 Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao...
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  Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your ResearchTypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...