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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: REV

Showing: 281 - 300 of 610 results

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...

  6. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...collaborator Some of these people you meet will review your papers or grants in the future, and don’t ... are talking to is really in your direct area.  Review the conference schedule and attendance list. Plan...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical signaling ...gradients for control of growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed...

  10. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...induce genome editing. Such a system has been previously described for transcriptional activation/repression...

  13. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ..., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along...

  20. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...to light as antibodies became commercialized, revealing the unreliability of antibodies. There are three...reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody crisis states...
Showing: 281 - 300 of 610 results