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  2. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

  3. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

  5. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...

  7. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...editing. Using several rapid and cost-effective screening methods, David Liu’s lab identified and validated...

  8. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

  9. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

  11. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators...

  12. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...

  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ... such as protein or DNA binding, enzyme or transcriptional activation, conformational changes, translation...

  14. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....

  15. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow...of biosensors could be used in high throughput screening for agrochemical compounds that have the potential...

  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

  17. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

  20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics ...
Showing: 281 - 300 of 606 results