We narrowed to 389 results for: control
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TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....operon. Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....
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3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... target of interest, you can add an additional control containing treated cells. The expected change in... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....conformational state of the HNH domain also allosterically controls RuvC activity through a hinge region between the...Kaplan, M., & Doudna, J. A. (2015). Conformational control of DNA target cleavage by CRISPR-Cas9. Nature, ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....second major area deals in post-experiment quality control, a good example is CRISPR-GA. These tools assess...place to focus further development toward. Quality control is, however, not a focus of this blog post. I separate... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....They might be researching enhancer elements that control the transcription of a gene a megabase away, or... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while having... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....respectively, are used as positive and negative controls for proper ER structure. Based on these data, ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin promoter. Purpose of this... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....protein. The expression of this fusion is under control of the arabinose-inducible promoter. Cells are ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments. Find the scrambled... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m.... the details, but the developers have it under control. 9:30 AM--Back-to-back meetings. Meet with my boss... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m...., you’ll want the proper positive and negative controls for your PCR. Find the details and learn more... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....sequence is also found in the reporter gene). A control RFP expression plasmid. With this set up, all... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....be sure to include proper positive and negative controls as you get your assays up and running. Ed.'s ... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....fluorescent tools are used simultaneously to monitor or control different cell types or signals in the brain. For... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....they worry about needing more study subjects and control groups. The important point, however, is that cost...