...conformational state of the HNH domain also allosterically controls RuvC activity through a hinge region between the...Kaplan, M., & Doudna, J. A. (2015). Conformational control of DNA target cleavage by CRISPR-Cas9. Nature, ...

...second major area deals in post-experiment quality control, a good example is CRISPR-GA. These tools assess...place to focus further development toward. Quality control is, however, not a focus of this blog post. I separate...

...respectively, are used as positive and negative controls for proper ER structure. Based on these data, ...

...Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin promoter. Purpose of this...

...They might be researching enhancer elements that control the transcription of a gene a megabase away, or...

...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...

..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more...

...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...

...protein. The expression of this fusion is under control of the arabinose-inducible promoter. Cells are ...

...system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external...

... the details, but the developers have it under control. 9:30 AM--Back-to-back meetings. Meet with my boss...

...shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled...

...domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu...

...be sure to include proper positive and negative controls as you get your assays up and running.  Ed.'s ...

...photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while having...

...they worry about needing more study subjects and control groups. The important point, however, is that cost...

...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

...Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

...neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements .../off genetic switch can be used for expression control. (c) Viral delivery methods include focused ultrasound...to be aware of before starting an experiment. Controlling expression As systemic capsids are… well, systemic...tissues and cell types. If desired, spatiotemporal control of transgene expression or activity can be achieved...

...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure...