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TypeBlog PostPublishedFeb. 13, 2020, 2:34 p.m....Aavery! Welcome to Addgene, Aavery! Original post: In December, we introduced you to Blugene’s new...
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Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....Darrell Kotton Find additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....the Addgene blog Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher...refresher on CRISPR with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....This post was contributed by Sreepadmanabh M, a member of the iGEM team from IISER Bhopal. Following ...the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids for your... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....microbiology blog posts Read about synthetic biology on the blog Find all of our Plasmids 101 blog posts Resources... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....Read all of our Plasmids 101 blog posts Check out all of our blog posts on plasmid cloning Resources on... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....details on acoustic reporters, check out this blog post. Bourdeau RW, et al., Nature. 2018. PubMed PMID:...miRNAs) are recognized as important regulators of post-transcriptional gene expression across the majority... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....Addgene blog Browse our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager...options Check out all of our science career blog posts ... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....yellow-green light (Figure 2). Despite the title of this post, this reaction is not fluorescence! Chemiluminescence...assay have you used? Share your tips below! This post was originally written by Jason Niehaus in June ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m.... specific restriction sites or DNA ligase! This post was originally written by Mary Gearing in December...Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....Nucleocapsid. WPRE Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that...poly A tract just after the R sequence. This post was written and updated by multiple Addgenies. It... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....information differently in a poster compared to an online abstract or Addgene blog post. Here, we are presenting...Clear and Accessible Scientific Writing.” In this post, we’d like to share parts of our workshop with you... scientific writing across all platforms, from posters to papers. We hope this helps, and happy writing... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m.... Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping to spread... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression...tag (dTAG). These control systems function at the post-translational level, unlike most of the other mechanisms... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...Plasmid Technologies Read Other Synthetic Biology Posts Suggest Plasmid Tools for the Repository Resources...with their environment. Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m.... Tips for Raising Startup Capital in Biotech - Posted on Forbes.com, contributed by Bruce Booth How to... Good Relations - PR for the Biotech Business - Posted on Nature.com, contributed by Julia Phillips and... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot...with any questions about mentoring. Read the other posts in the series to get more advice on mentoring: ...group In my travels speaking to grad students and postdocs I have started to talk to trainees more about ...and career stage (e.g. all women, all men, all postdocs, all grad students) or mixed for one or both of... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....kicking it off by sharing all the incredible guest posts and stories featured in our blog in 2023. Some of... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post. On the twelfth day of CRISPR, Addgene gave to ...