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Showing: 281 - 300 of 694 results

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...the Addgene blog Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher...refresher on CRISPR with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find ...

  3. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...This post was contributed by Sreepadmanabh M, a member of the iGEM team from IISER Bhopal. Following ...the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids for your...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...microbiology blog posts Read about synthetic biology on the blog Find all of our Plasmids 101 blog posts Resources...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Addgene blog Browse our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ... specific restriction sites or DNA ligase! This post was originally written by Mary Gearing in December...Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing ...

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Nucleocapsid. WPRE Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that...poly A tract just after the R sequence. This post was written and updated by multiple Addgenies. It...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression...tag (dTAG). These control systems function at the post-translational level, unlike most of the other mechanisms...

  12. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...information differently in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting...Clear and Accessible Scientific Writing.” In this post, we’d like to share parts of our workshop with you... scientific writing across all platforms, from posters to papers.  We hope this helps, and happy writing...

  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the Repository Resources...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot...with any questions about mentoring. Read the other posts in the series to get more advice on mentoring: ...group In my travels speaking to grad students and postdocs I have started to talk to trainees more about ...and career stage (e.g. all women, all men, all postdocs, all grad students) or mixed for one or both of...

  16. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post.  On the twelfth day of CRISPR, Addgene gave to ...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory...genes involved in a phenotype of interest. This blog post will discuss differences between these approaches...CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple knockout...M. Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation...Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models Learn about Homology Directed...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...results. But what is appropriately validated? This post will go over some of the general considerations ...antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications... Antibodies 101: Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding...
Showing: 281 - 300 of 694 results