We narrowed to 842 results for: Eras

...One of the biggest barriers to gene therapy treatment of central nervous system (CNS) diseases is the...because in vivo transduction of AAV serotypes generally tracks between strains of mice. This suggests ... engineered PHP.B. PHP.B was created by first generating a diverse library of AAV9 mutants that had a ...inheritance pattern of PHP.B permissiveness. The first generation of outbred mice (F1) had half the level of CNS...transduction as their C57BL/6J parent. The second generation (F2) of outbred mice also showed a Mendelian ...galactose and AAVR to transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they ...Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613. Hordeaux...

... We’ve previously discussed some of the general considerations for performing behavioral tests, but now... modulating. Viral vectors, in contrast, are considerably more versatile and specific in how they manipulate...field box or a circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex... of individual neurons and in terms of their interactions with one another. In addition to the neuronal...controlling muscle activity and coordinating movement, several additional brain areas that reflect motivational...sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests...rodents can be used to study anxiety levels in general, or anxiety associated with psychiatric diseases...

...that stably express a gene of interest. While the general approach for using lentivirus, infect and select...consider is the age of your culture post thaw. In general, the higher the passage number of a culture the...routinely test for mycoplasma contamination. There are several commercially available mycoplasma detection kits...parameters. For example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus...your target cells Labs tend to follow the same general procedure for lentiviral infections, produce the...earliest steps in lentiviral transduction is the interaction between the viral envelop and the receptors on... on the target cell surface. The possible interactions and hence possible targets for a lentivirus are...

...in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies...therefore, critical to understand how proteins interact with one another (or with DNA) to perform diverse...the most popular approaches to study protein interactions is the pull-down assay, wherein a protein of...analysed by mass spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin...however, to these IP-based assays is to find (or generate in-house) a pre-validated antibody for each target...throughput because numerous cell lines can be generated, each with a different tagged protein of interest...these factors prevent stem cell researchers from generating clonal knock-in lines, especially for ambitious...

...enhancers, and cross-cassette interactions in gene expression, and to generate multi-gene knockouts. Mammalian...MoClo background MoClo is a clever way to assemble several DNA parts in a specific orientation using Type ...2011). It can be performed at multiple levels to generate anything from individual transcription units (...products without the undesirable side products generated during chemical synthesis of these molecules. ...bacteria. If your yeast experimental goals are more general in nature, or you aren’t planning on using K. phaffii...Multiplex Yeast Toolkit builds on the YTK and adds several additional capabilities, including conditional ...., 2014). This kit is a great starter for your general plant cloning experiments. Addgene also has two...

...photobleaching. Interaction with receptors: Understanding how fluorescent dyes interact with specific receptors...intermolecular FRET for ligand-receptor interactions, require specific considerations regarding the choice of fluorescent...crucial in the world of biological research, medical therapy, and even drug development (Grimm & Lavis, 2022...targets through fluorescent labeling. There are several advantages to using these self-labeling proteins... is bound through green light emission. Key considerations in selecting fluorescent dyes/ligands Effects...best practice to determine optimal DOL is through several small-batch experimental labeling. Size is also...under certain conditions, which is an important consideration for assay design (Breen et al., 2016). When ...

... for Autoinhibition Release of Methyltransferase (CHARM), with several advantages over the earlier system...and stimulate the endogenous methyltransferase. The strategy is generalizable to a variety of DNA-targeting...Arimura et al. used MagIC-cryo-EM to determine several structures (at < 5 Å resolution) of the linker ....    Figure 2: A) When proteins (blue) interact with the air-water interface (AWI) before vitrification... 2024). The best-performing variant yielded an average integration efficiency of 23%, a more than four-fold...editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional silencing...  Rather than overexpressing an exogenous methyltransferase for epigenetic silencing, which can be toxic...

...Chamberlain JS (2011) Gene Therapy of mdx Mice With Large Truncated Dystrophins Generated by Recombination Using... reassemble fragmented genomes is the basis of several split vector approaches developed in the early ...full-length transgene (Chamberlain et al., 2016). It’s generally accepted that some form of homologous recombination...exact mechanism is not yet known. Drawbacks:  Generally an overlapping split vector has equal or higher...is currently no one ideal split vector system. Overall the efficiency of transgene expression from split... of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...Capacity in a Transgene-independent Manner. Molecular Therapy 16:124–130 . https://doi.org/10.1038/sj.mt.6300322...

...mouse inner ear. Fourth-generation cytosine base editors The fourth-generation base editors, BE4, reduce...variety of targets in human cell lines, with an average indel frequency of only 1.1%. These numbers are...over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was only 0.5%, and...18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1,...these three improvements represent the fourth generation of base editors. Compared to BE3, BE4 offers ...ABE7.10 is the most active editor, displaying an average editing efficiency of 53% with an editing window... phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which edits ~590-fold faster...

.... To overcome this limitation, we created a generalizable tag-based strategy, which we refer to as the... target degradation (Figure 1). Plasmids: We generated a series of plasmids that are available from Addgene...of N- or C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V. Detailed...or Jay Bradner ([email protected]). Considerations when getting the dTAG system up and running...your lab Confirm the functionality of your fusion chimeras For each protein-of-interest, it is important ...FKBP12F36V tag without disrupting protein function. A literature search can help determine which terminus is favorable... evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines we have evaluated...