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We narrowed to 322 results for: Mut

Showing: 151 - 160 of 322 results
  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...gene. One such strain is DB3.1, which contains a mutant version of DNA gyrase (gyrA462) that is resistant... al. “F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic proteins which retain ...
  2. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...with BioRender.com. Add silent mutations near point mutations to create 3-base (or longer) tracts...
  3. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... if you want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region... hand, if you are trying to knock-in a patient mutation or need to make a precise amino acid change, there...
  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...co-injection of Cas9 and a single-guide RNA targeting the mutant allele. When all was said and done, the researchers...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...
  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed...
  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...additional SapTrap plasmids used for introducing point mutations that was published in a microPublication (Schwartz... E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication 1...
  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...orientation limiting FRET efficiency, circular permutation (Baird et al., 1999) of the fluorescent protein...References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion within green fluorescent...
  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as well as any...functional consequences of that change. For example, “Mutated aspartate 123 to Lysine (D123K), lowers biosensor...
Showing: 151 - 160 of 322 results