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  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... their restriction sites allowing you to easily create customized overhangs to assemble up to 6 different...number of fragments you’re hoping to assemble increases. Multiple inserts: There are some cloning methods...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and...have a kit!). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...learning from the careers of others were key to creating my career path. Luckily, in grad school much of...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  6. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...students, postdocs, and other working scientists, creating a presentation for 8th graders was a little intimidating...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...gRNA.  While this methodology has proven to be incredibly valuable to scientists, there are some potential...Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...researcher experiences and that it often provides incredibly valuable insight into one’s work. As a community...information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance...

  9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...epitopes previously hidden within the protein. Created with BioRender.   Permeabilization What is ...better at permeabilizing organellar membranes. Created with BioRender.   How do I choose?! Ok, so ...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...EVER confirmed this with me). While things were incredibly busy in those early years, the founders knew ...aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze for all of...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of survival. This...antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies, and investigate more...

  14. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...gene disruption in Mycobacterium abscessus, an increasingly common human pathogen notorious for its high...adhesion to a PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service...GCaMP6. It could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...the presence of your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or ...non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform called DETECTR. How does...

  18. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... you when you need it. The opportunity to be creative and think critically. One of the very first times...defined as sitting down at a computer and just creating, which for many scientists is the most enjoyable...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  21. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

  22. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

  23. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...

  24. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins...

  25. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

  27. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

  28. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

  29. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

  30. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you..., Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for ...

  32. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...

  33. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...

  35. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...

  36. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...

  37. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...

  38. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

  39. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...

  40. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...
Showing: 601 - 640 of 788 results