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Showing: 751 - 778 of 778 results

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PCR Applications for Copy Number Variation Assays

Type

Blog Post

Published

April 26, 2022, 1:15 p.m.

...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

Published

Feb. 4, 2016, 3:30 p.m.

...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...
An Introduction to Adenovirus

Type

Blog Post

Published

Oct. 3, 2023, 1:15 p.m.

...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...
Tips for arabidopsis transformation

Type

Blog Post

Published

Oct. 25, 2018, 1:23 p.m.

...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

Published

Oct. 4, 2018, 12:44 p.m.

... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...
Great Results Start with Great Standard Curves

Type

Blog Post

Published

Aug. 15, 2024, 1:15 p.m.

...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

Published

Sept. 28, 2017, 2:01 p.m.

...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

Published

Dec. 7, 2020, 2:53 p.m.

...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

Published

March 17, 2016, 2:30 p.m.

...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

Published

Aug. 20, 2020, 2:30 p.m.

...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

Published

July 7, 2020, 1:15 p.m.

...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...
CRISPR-mediated Plant Base Editors

Type

Blog Post

Published

Jan. 3, 2019, 1:35 p.m.

... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

Published

Nov. 1, 2018, 12:35 p.m.

...through a second recombination event between the two flanking repeats. In cells where recombination has not ...
Hot Plasmids: Fall 2024

Type

Blog Post

Published

Nov. 21, 2024, 1:30 p.m.

...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...
How to Write a Scientific Review Article

Type

Blog Post

Published

Feb. 16, 2017, 3:30 p.m.

...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

Published

July 2, 2019, 12:51 p.m.

...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...
Plasmids 101: Control Plasmids

Type

Blog Post

Published

April 9, 2015, 3:29 p.m.

...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...
CRISPR 101: Homology Directed Repair

Type

Blog Post

Published

Jan. 26, 2023, 2:15 p.m.

.... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

Published

Oct. 11, 2016, 2:30 p.m.

... you chose for your experiment, but, if you’re working with plants, don’t be afraid to try your hand at...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

Published

Oct. 5, 2021, 1:15 p.m.

...lamps are better, and xenon lamps are the best for working in this spectral range. You can also check that...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

Published

Jan. 26, 2016, 3:30 p.m.

...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

Published

April 5, 2018, 12:42 p.m.

...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...
Plasmids 101: Restriction Cloning

Type

Blog Post

Published

Feb. 18, 2016, 3:42 p.m.

... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

Published

Oct. 17, 2019, 12:28 p.m.

...inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed...
Antibodies 101: Validation

Type

Blog Post

Published

March 24, 2022, 1:15 p.m.

...general suggestions given by the International Working Group on Antibody Validation (Uhlen, et al., 2016...
Antibodies 101: Introduction to Antibodies

Type

Blog Post

Published

Jan. 19, 2021, 2:15 p.m.

...green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing an inflammatory...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

Published

Nov. 6, 2018, 1:12 p.m.

...induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID...

Showing: 751 - 778 of 778 results

Results per page:

PCR Applications for Copy Number Variation Assays

Anatomy of a Plasmid Page at Addgene

An Introduction to Adenovirus

Tips for arabidopsis transformation

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Great Results Start with Great Standard Curves

AAVs CREATed for Gene Delivery to the CNS and PNS

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

CRISPR-mediated Plant Base Editors

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Hot Plasmids: Fall 2024

How to Write a Scientific Review Article

New Tools Enable CRISPRa for Neuroscience Applications

Plasmids 101: Control Plasmids

CRISPR 101: Homology Directed Repair

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Fluorescent Proteins 101: When GFP lets you down

Quick Guide to Near-Infrared Fluorescent Proteins

Treating Muscular Dystrophy with CRISPR Gene Editing

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Plasmids 101: Restriction Cloning

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Antibodies 101: Validation

Antibodies 101: Introduction to Antibodies

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

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