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We narrowed to 830 results for: Eras

Showing: 301 - 320 of 830 results

  1. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...lab in Harvard's Department of Stem Cell and Regenerative Biology is to understand the basis for cardiovascular...use their TALEN kit to quickly and efficiently generate human stem cells edited to carry mutant versions... In the Cell Stem Cell paper, for example, we generated hepatocytes to examine the effect of a gene on... metabolism. The TALEN kit is quite useful in generating the mutant cell lines that one needs to do disease.... That technology had been used for decades to generate knock-out mice, but for various reasons it is ...sequence a large number of individuals and find several mutations in the same gene or in multiple genes...Ding et al. "A TALEN genome-editing system for generating human stem cell-based disease models." Cell Stem...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...exciting revelation. Science in any field can generally be applied to improve our lives and solve global...scientific process can help us understand how new therapies can be developed and applied. The current pandemic...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...environment and with supportive mentors, being open and vulnerable about my struggles and what I don’t know yet ...also found that it is more common among first generation students, women, BIPOC, and other minoritized...like these can be highly beneficial. I’ve made several close friends and found incredible mentors through...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the..., 2019). They called their method Metagenomic Alteration of Gut microbiome by In situ Conjugation (MAGIC...vitro and in vivo. They identified cells from 19 genera across four phyla that have taken up the plasmid...vivo studies. However, they only identified seven genera in in vitro experiments, possibly because in vitro...one cell during cell division. To achieve the agglomeration of fluorescent proteins into one particle within...decreases over time and the number of elapsed generations can be determined. The lab used DCDC to measure...measure growth rate of E. coli for over 10 generations and showed that this method can be used for both in...

  4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper ...the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural affinity and...higher order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or...homodimers or higher order oligomers can be analyzed by several techniques. Gel filtration and SDS-PAGE under non-denaturing...reflect the likelihood that fluorescent proteins interact or oligomerize in cells? This question is largely...fluorescent protein’s propensity to homodimerize. Several studies have used the OSER assay to demonstrate...

  5. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... Overview of the CRISPR classification hierarchy. The hierarchy flows from Class to type to subtype to...the array of CRISPR technologies. Learning the general principles of this classification system will help...classifications were placed into a fairly simple hierarchy: class to type to subtype to variant. Despite ...seemingly complicated steps to arrive at this hierarchy, the main aspects of each class and type are easy...Class 1, and are the most common type of CRISPR overall. Type I systems degrade large sections of DNA thanks...most common. Figure 4: Overview of the general CRISPR type VI mechanism. The Cas13 effector binds...diversity of rare CRISPR-Cas systems with deep terascale clustering. Science, 382(6673). https://doi.org...

  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...churn out antibodies against the injected protein. Several weeks later, blood from the animal is drawn and...antigen binding, the variable regions alone can be generated as a fusion protein. An ScFv is made up of the...your samples to a standard curve. This curve is generated using a serial dilution of a known amount of protein...for other experiments to study protein-protein interactions. Flow cytometry Flow cytometry is used to detect...2014.   In vivo uses For both research and therapeutic purposes, antibodies can be used to affect cellular...functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies can bind to and inhibit...

  7. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Performance dCas9-VP64 activation is generally thought of as the “first generation” CRISPR activator. While it ...initially published in 2013 (Bikard et al., 2013, Perez-Pinera et al., 2013). In the years that followed, innovative...Performance SunTag performs better than first generation activators while showing lower activation levels...to recruit transcription factors. Performance Generally, VPR is found to have significantly higher activation...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein...

  8. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...specific to the acceptor In order for FRET to occur several constraints must be met. The donor and acceptor...appropriately for energy transfer via dipole-dipole interaction. The efficiency of FRET can be measured for a...FRET range, FRET efficiency is used to infer an interaction between the fluorophores and serves as a small-scale...As the choice of fluorophore pairs is a key consideration for a FRET experiment, the possibilities can...of fluorescent proteins available. Fortunately, several excellent reviews have collated data from various... typically used for studying protein-protein interactions by measuring the change in FRET efficiency, ..., FRET experiments can probe protein-protein interactions, measure the concentration or activity of small...

  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...For example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...are multigene systems whose protein products can interact with the DNA that encodes the protein itself or... or other genes within the circuit. These interactions yield a cellular phenotype, translational output...can support a larger cellular engineering goal, several of which are described below. Multigene circuits...promoter for a given peptide can easily be tested by generating Level 1 TUs with unique promoters, and the ideal...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...your study is aimed at developing human therapies. If a therapeutic is the end goal, it is best to work with...Targeting is also important in the development of therapies when expression must be restricted to specific... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...ligands. However, they retain their intracellular interactions and still set off the same pathways, giving ... (Saloman, JL, et al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived ...& Gold, M. S. (2016). Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, as...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... In this article we will discuss general viral safety considerations and best practices for handling lab-grown... basic biological research as well as clinical therapy. These biological tools are useful because they... separate plasmid (envelope plasmid), and the polymerase (Pol) for replication and Gag structural protein... and the same holds true for types of viruses generated in the lab. The four most common types of viruses... lab and require additional safety measures.   General viral handling safety   Containment and personal...resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new tools...over the years, several alternative antibodies against the Myc tag have been generated in an attempt to.... However, it was (and still is) infeasible to generate highly specific antibodies for every potential...would help the field explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic...epitope within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer... Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020....synthetase complex, the team investigated whether the generated antibodies could bind these aaRSs. They found ... Gräslund lab plans to continue their work by generating antibodies for additional aminoacyl tRNA synthetases...nanoparticle can protect proteins from unfavorable interactions in the cell, improving biosynthetic pathway ...represent 0.001% of the population.  Because indel generation by Cas9 can occur at a steady rate, scientists... Farm GENIE project have arrived! We now offer several versions of GCaMP8 vectors in serotypes AAV1 and...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...carbon sources such as glycerol or glucose (in moderation) may also serve to increase cell density and ...plasmid yield. You can optimize your growth time, temperature, and shaking speed to maximize cell density and... and plasmid yield as these factors are generally, but not perfectly, correlated. Typical growth times...sufficient gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around...plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work for every plasmid...plasmid Certain plasmids and strains grow best at temperatures other than the standard 37℃. This plasmid feature...recommendations to grow a plasmid at 30℃ or room temperature. These recommendations also often require a longer...

  15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...describe and interact with the world using words like OR and AND. These logical operators are so useful...collection is the beginnings of what we think of as an operating system for engineering an entire cell. Typically...BIOFAB project is seeding the beginnings of an operating system for programming life, starting with the... pieces in these kits as the beginnings of an operating system or language for programming life. Which...as applied to products on the domestic market generates $300 billion dollars every year. That's two percent...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial tests, Cas13b...Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average knockdown, and they chose this enzyme for further...editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also...and designated this system REPAIRv1. Using next-generation sequencing, they confirmed A->I editing and found...off-target activity. By mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

  17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE, and... lab places an order, they are likely to order several plasmids at once, breaking the statistical independence...our distribution, resulting in the following hierarchical model: Because the U.S., Europe, and China ...the inferred percentage of normal activity (the average rate of unique PIs/labs placing orders per day)...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...

  18. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...size of bacterial culture you’re working with. Overall, the glass syringe filter method is not only cheaper...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR Purification...

  19. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...company that is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. ...s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta, an early-stage biotech company...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...

  20. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start... of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and other enzymes...fluorescent proteins, antibiotic resistance, and luciferase. References Gaudelli NM, Komor AC, Rees HA,...CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038...
Showing: 301 - 320 of 830 results