We narrowed to 570 results for: FIE

...Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding... be used to modify genomic loci that cannot be modified using wild-type SpCas9. The number of off-target...

...sequence-specific protease used to cleave affinity tags from purified recombinant proteins at small and large scale....biotinylation. Once induced, the NBs could be easily purified by one-step immobilized metal ion adsorption chromatography...

...editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies...reporters- eGFP L202, eGFP L138, and eGFP L93. The modified eGFPs were placed downstream of wild-type mCherry...

...Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues had moved...effort in the future to match people within similar fields (ASM Microbe has eight tracks), something we didn...

... microscopy, GVs visible in cells via TEM, and purified GVs under TEM. (c) Ultrasound imaging of tumor...231 cells (top, scalebar = 10µm) and TEM of GVs purified from MDA-MB-231 cell lysate after 4-days of expression...

...into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated it into a...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...

...method with Agrobacterium: If you are new to the field, the following are useful tips for your first transient... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...mouse (Zhou et al., 2018). This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits...

... was necessary and reasonable. PLOS has since clarified their data sharing policy and recently announced... PLOS’s credit, the organization subsequently clarified what the policy intent was and provided an FAQ...

...with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), magnetic bead...methyltransferase DNMT3A to the genomic location specified by the DNA-binding protein. The ADD domain of ...

...vitro by measuring the fluorescence intensity of purified protein at different acid concentrations. Overviews...Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

...from general note taking tools, to more scientific field-based solutions or open source solutions. In comparison...of budget and ease of use of the software. We identified that when talking about ELN costs and limited...

... over millimeter-long distances across multiple fields of view, whereas previous Brainbow technologies...useful for discriminating neurons within only one field of view. Tetbow can be introduced in vivo either...

...virtually all kinds of experiments and research fields, for any experiment, your first three gates will.../SSC gate.   Single-cell gating Once you have identified and gated on your cells of interest, you will...

...you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

...collaborators, others you know in the field, and even people who aren’t in the field. One PI on the panel even suggested...

...materials, and resources that you’ve thoroughly identified. Do online searches, and seek advice from your...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

...utilized HiBiT to explore TP53 biology. They first identified that in a set of patients with gastric cancer...-HiBiT in cell lysates, conditioned media, and purified EV fractions (Shibata et al., 2023). Luminescence...

...leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management...

...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...