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We narrowed to 566 results for: FIE

Showing: 301 - 320 of 566 results

  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated it into a...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...and attR sites.   Gateway vectors contain modified versions of the att sites so that scientists can...thanks to the Gateway vectors’ design. They have modified versions of the attB, P, L and R sites that recombine...

  3. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...sequencing also relies on sequencing by synthesis, but modifies the original Sanger procedure to improve throughput... base identity and length, but disregards the specified discrepancies. Any sequences that are a perfect...

  4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...process to produce a multigene construct. Figure exemplifies the extraction and use of a coding sequence of...

  5. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...quantities of the target!) and use them in numerous fields including research and development, diagnostics...could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control will...

  6. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... microscopy, GVs visible in cells via TEM, and purified GVs under TEM. (c) Ultrasound imaging of tumor...231 cells (top, scalebar = 10µm) and TEM of GVs purified from MDA-MB-231 cell lysate after 4-days of expression...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...from general note taking tools, to more scientific field-based solutions or open source solutions. In comparison...of budget and ease of use of the software. We identified that when talking about ELN costs and limited...

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), magnetic bead...methyltransferase DNMT3A to the genomic location specified by the DNA-binding protein. The ADD domain of ...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...virtually all kinds of experiments and research fields, for any experiment, your first three gates will.../SSC gate.   Single-cell gating Once you have identified and gated on your cells of interest, you will...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

  13. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...vitro by measuring the fluorescence intensity of purified protein at different acid concentrations. Overviews...Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...materials, and resources that you’ve thoroughly identified. Do online searches, and seek advice from your...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...mutating a specific amino acid or introducing a specified edit). However, if your design does have wiggle...depend on a DNA mutation, off-targets must be quantified slightly differently. For a comprehensive approach...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...assay, including examples of RBPs successfully identified using this method. Step 1: Prepare a random fragment...Elisabeth A. Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical...

  19. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management...
Showing: 301 - 320 of 566 results