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Showing: 301 - 320 of 553 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...vitro by measuring the fluorescence intensity of purified protein at different acid concentrations. Overviews...Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...from general note taking tools, to more scientific field-based solutions or open source solutions. In comparison...of budget and ease of use of the software. We identified that when talking about ELN costs and limited...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...materials, and resources that you’ve thoroughly identified. Do online searches, and seek advice from your...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

  7. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...mutating a specific amino acid or introducing a specified edit). However, if your design does have wiggle...depend on a DNA mutation, off-targets must be quantified slightly differently. For a comprehensive approach...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management...

  10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...assay, including examples of RBPs successfully identified using this method. Step 1: Prepare a random fragment...Elisabeth A. Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use the same tag...649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with different dyes. b)...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...Lentivirus Production and Transduction Protocols   Once purified, you must titrate your viral particles to determine...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...sets. Significance is a numerical value which quantifies the likelihood that a result is due to chance...Benjamini uses a complementary approach which identifies a p value cut off. This method entails arranging...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...the key components of transposons, how they are classified, and how they are used in the lab. What is a ...Figure 2: Overview of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize...

  16. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...then you expect that the bulk of the peptides identified to be from the target protein. This approach ...the majority of the protein in the sample was identified as your target protein, allowing you to proceed...

  17. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... grad school, you searched for and eventually identified a postdoctoral mentor. A good mentor typically...yourself to and interact with the members of your field, and present your research. Face to face meetings...

  18. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea... Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA...

  19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...cells (9). PA-FPs have helped to revolutionize the field of super resolution microscopy. PA-FPs, especially...reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6...

  20. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... use antibodies for experiments in a variety of fields including neuroscience and immunology. They can... the antibody using a low-pH buffer. Then, the purified protein can be used in experiments to compare ...
Showing: 301 - 320 of 553 results