We narrowed to 383 results for: FLU

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

2. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...the person as well as their current sphere of influence. In other words, the longer the recommender knows...

3. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...each other. Chromatin regulation is complex and influences--directly or indirectly--many other areas of ...

4. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

5. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

6. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ... normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing RecA and...

7. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   Published

   May 22, 2014, 4:02 p.m.

   ...should also be published. All these factors will influence your choice of journals to submit your work to...

8. Antibodies 101: Fab Fragments

   Type

   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem...

9. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...disparities might suggest underlying sex-based influences on physiologic or pathologic processes. Much ...

10. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...Addgene’s blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...

11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...channelrhodopsins respond by opening and allowing an influx of ions into the neuron which generates an action...

12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ... assess the potential impact of these salary fluctuations, we are continuing to reach out to institutions...

13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...flags about how the work was performed that could influence the results? The peer-review process is not perfect...

14. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate ion...

15. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...to edit The sequence you choose to install can influence the success of your prime edit. If possible, consider...

16. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...

17. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression...

18. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and...

19. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do NOT change ...

20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...partners other than those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify...