We narrowed to 354 results for: HRE

1. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ... plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed by sacB counterselection...

2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ... to be edited. In this blog post, we will cover three ways to circumvent this limitation: 1) the use of...

3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue), which...

4. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ...easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV...

5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ...duplicated (i.e., redundant) genome, their long three-month generation time and labor-intensive maintenance...

6. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ... recently who referred to her lab as her family three times during a 10 minute conversation. Leaving a...

7. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...C mutations in the plant genome. We constructed three versions of the vector (pKABE6.3, pKABE7.9, and ...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ... just described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin...

9. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...solution. These characteristics give us information on three important factors. What can AAV titers tell you?...

10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology...

11. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...Fortunately I have a lot of links and content at hand in three useful places: I had posted content on many of ...

12. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...want to activate, you should also be ready to test three or four guides directed towards that gene as there...

13. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...probed with β-actin (1:2000). Representative of three independent blots. B–C) Graphs showing the relative...

14. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...better, we can use the same fluorophore for the three lineage markers we have chosen. As a result, all...

15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...Lab used such a strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development...

16. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...sister chromatid, plasmid, etc.), and consists of three pathways: classical double-strand break repair (...

17. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to the use of antibodies to...

18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...domain. They can also be used in crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova...

19. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...about possible unintended consequences of a march. Three of these risks include: 1) Lost trust Perhaps the...

20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...protection, publications and funding. If any of these three pillars crashes, the lab's success and the careers...