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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 736 results for: NSI

Showing: 301 - 320 of 736 results

  2. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...caspases are activated last in the cascade and are responsible for the degradation of over 600 cellular components...detected fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu ...developing forebrain and retina. This pattern was consistent with TUNEL staining, a popular method to observe...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address...required for the transduced cell to become light responsive than fluorescent proteins required to get a good...interior, causing less particles to be released into suspension and lowering the number of viral particles that...

  4. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...space where BosLab exists today. Back then, it consisted of “wooden slab benches” set on top of filing ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming...biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules....

  5. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... institutes, the Tech Transfer department is responsible for managing research use agreements like MTAs...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

  6. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright...than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light and electron...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains...

  7. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

  8. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...meetings tend to fill the shape of their container. Consider whether you actually need a full hour to achieve...FInd Tips on Picking a Research Project Read Other Inside Addgene Posts Resources on the Addgene Website...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... perhaps surprising, yet important, factors to consider when seeking your dream lab. The principal investigator's...want a non-academic career path?  If you are considering this, make sure to choose an advisor that supports...where the topic of your research can make your transition easier – choose a lab that works on problems ...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... Temperature inducible promoters Temperature sensitive expression systems are typically less leaky than...Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light is absent, YFI phosphorylates...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...

  11. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...this solution wasn’t practical due to the low intensity of luminescent proteins. To make luminescent imaging...luciferase has a low quantum yield, and when transiently expressed in cells, generates much less signal...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

  12. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured...did! From Addgene's very beginning, we had to consider digital accessibility for our website and online...

  13. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... mammals, which is thought to be due to their propensity to integrate into proto-oncogenes and disrupt...alternative virus, or other strategies may need to be considered if size constraints are an issue. Adenovirus...Adenovirus is an effective option if you need high, transient expression of a genetic cargo or have a very large...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ... lower background levels and improved signal sensitivity. Another advantage: luciferase reporters aren...Not required Signal strength Lower Higher Sensitivity Higher Lower Background Lower Higher Photobleaching...change in conformation of a single protein. Considerations for luciferase assays Luciferases can only ...

  15. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...repair template (sister chromatid or homologue) or extensive DNA synthesis. NHEJ also finishes repair of most...streamlined version of this pathway and does not consider the missing or damaged nucleotides that are common...Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References...

  16. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...you are spending on a conference? One thing to consider is the size of the conference. Large conferences...favor small conferences where the attendees stay onsite and eat together because scientists have time to...– even students and postdocs. There are many inexpensive/free places to order cards. There are a few good...

  17. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...publication in a scientific journal requires several considerations. First, it is important to establish the type...journal. An increasing number of funding bodies insist that the research data resulting from their funding...is a Developmental Editor for F1000Research, responsible for assessing the scientific content of manuscripts...

  18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent... Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibody, this approach can greatly increase the sensitivity of an assay by increasing the number of signaling...place over multiple days. Increased run time is considered one of the major drawbacks of the indirect method...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...advantage of polyclonal antibodies is that they are inexpensive. Their short production time and the bulk purification...labs. Polyclonal antibodies also have a high sensitivity to the target protein due to their ability to...or fixation (IHC). Because of their increased sensitivity, polyclonal antibodies are particularly useful...
Showing: 301 - 320 of 736 results