We narrowed to 746 results for: NSI

...cells. Until now, tools for doing this were labor-intensive and required using manipulations such as injection...sites in the construct to different lengths, the density of cells that are labeled can be altered to dense...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

...a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...

...markers and replication origins, but there is extensive heterogeneity in the feature sequences due to ...control elements in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to...reliable feature annotations. Even with these extensive efforts, such an algorithm has limitations. For...

...space where BosLab exists today. Back then, it consisted of “wooden slab benches” set on top of filing ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming...biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules....

... institutes, the Tech Transfer department is responsible for managing research use agreements like MTAs...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

...then integrates into the genome. Thus the gRNAs responsible for a phenotype can be identified by next-generation...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...a requirement for very high excitation light intensities have made these indicators difficult to work ...

...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

...almost complete desensitization when exposed to continuous bright light. Desensitization is mediated by...discovered Marine, Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that...

...caspases are activated last in the cascade and are responsible for the degradation of over 600 cellular components...detected fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu ...developing forebrain and retina. This pattern was consistent with TUNEL staining, a popular method to observe...

...applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright...than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light and electron...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains...

...using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address...required for the transduced cell to become light responsive than fluorescent proteins required to get a good...interior, causing less particles to be released into suspension and lowering the number of viral particles that...

...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...meetings tend to fill the shape of their container. Consider whether you actually need a full hour to achieve...FInd Tips on Picking a Research Project Read Other Inside Addgene Posts Resources on the Addgene Website...

... perhaps surprising, yet important, factors to consider when seeking your dream lab. The principal investigator's...want a non-academic career path?  If you are considering this, make sure to choose an advisor that supports...where the topic of your research can make your transition easier – choose a lab that works on problems ...

...this solution wasn’t practical due to the low intensity of luminescent proteins. To make luminescent imaging...luciferase has a low quantum yield, and when transiently expressed in cells, generates much less signal...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

... mammals, which is thought to be due to their propensity to integrate into proto-oncogenes and disrupt...alternative virus, or other strategies may need to be considered if size constraints are an issue. Adenovirus...Adenovirus is an effective option if you need high, transient expression of a genetic cargo or have a very large...

... lower background levels and improved signal sensitivity. Another advantage: luciferase reporters aren...Not required Signal strength Lower Higher Sensitivity Higher Lower Background Lower Higher Photobleaching...change in conformation of a single protein. Considerations for luciferase assays Luciferases can only ...

...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured...did! From Addgene's very beginning, we had to consider digital accessibility for our website and online...

... Temperature inducible promoters Temperature sensitive expression systems are typically less leaky than...Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light is absent, YFI phosphorylates...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...

...repair template (sister chromatid or homologue) or extensive DNA synthesis. NHEJ also finishes repair of most...streamlined version of this pathway and does not consider the missing or damaged nucleotides that are common...Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References...