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TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....successes of retinal gene therapy, an ever-growing number of clinical trials for devastating genetic diseases...
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Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....bands or a few strong ones, consider increasing the number of washes performed during your harvest. Validation... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m..../or a 3x cysteine varieties. This refers to the number of cysteine sites the nanobody has available for... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....step, the final construct can contain an unlimited number of modules. Constraints on the order of the modules... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo therapy... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....homology-directed repair (HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....development targeted to the grants profession? The number of organizations, both public and private, that... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....SIN. Packaging plasmid(s) contain the minimal number of HIV genes required for virus production (3 or... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m.... bacteria in favorable conditions double their numbers rapidly. The knowledge the Singletons gained from... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....requires a very specific PAM sequence that limits the number of targetable loci, and truncated Cas9 has much... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....and Eric Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....base editor platforms. Hua et al. constructed a number of plant base editors replacing the SpCas9 with... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....here is only a small selection of the increasing number of studies using viral vectors in behavioral neuroscience... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....dropped” in the wrong place, while increasing the number of potential applications. But they weren’t done... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m..... PE7 shows enhanced editing efficiencies in a number of cell types at different genomic loci — in some... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m.... thing I did was stare in horror at the limited number of hits. So, here, I will share my process with... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....fits your experiment. This choice will depend on a number of factors, like your organism, desired expression... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....cells in real time. Previously, we have described a number of practical considerations that should be taken... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....expression strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed...