Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 610 results for: REV

Showing: 301 - 320 of 610 results

  1. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...to light as antibodies became commercialized, revealing the unreliability of antibodies. There are three...reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody crisis states...

  2. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

  5. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...based methods for viral titration consider using a reverse transcriptase mutant in a separate control reaction...over-inflate results. A packing vector deficient in reverse transcriptase will produce low levels of virus ...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... histones, the more compacted it becomes. This prevents the cellular machinery from physically interacting... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding...

  7. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...need to be signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it ...items. This will give your supervisor a chance to review what you have discussed and add suggestions if ...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...photostability — 25-fold over mVenus and 4-fold over the previous best performer mGold — while maintaining high ... Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...These benefits of Cas3 do come at a cost — the previously mentioned issues with expressing all Cascade ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological systems...exist at all. Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  19. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to...

  20. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... lab, or by a previous group. If the plasmid was made by a modifying a vector previously deposited with...
Showing: 301 - 320 of 610 results