We narrowed to 610 results for: SCR
-
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...
-
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....ready-to-use viral preps Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...University Salk Institute for Biological Studies The Scripps Research Institute St. Jude Children's Research... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org ... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based ...expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal amount ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics ... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature. “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....promoter regions that activate fluorescent protein transcription in response to river contaminants and transform...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m.... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....directly transformed into E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries...Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on ... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...