We narrowed to 389 results for: control

1. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

2. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

3. Viral Vectors 101: Systemic Capsids

   Type

   Blog Post

   Published

   Oct. 12, 2023, 1:15 p.m.

   ...neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements .../off genetic switch can be used for expression control. (c) Viral delivery methods include focused ultrasound...to be aware of before starting an experiment. Controlling expression As systemic capsids are… well, systemic...tissues and cell types. If desired, spatiotemporal control of transgene expression or activity can be achieved...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian...

5. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic the natural...

6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...this is indicative of protein degradation). To control for protein degradation due to other pathways, ...

7. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...PubMed PMID: 1324324. 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989...

9. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...decrease the expression of TAK 1, an enzyme that controls apoptosis and regulates transcription. Without... cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly” microenvironment...

10. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure...

11. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

12. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points...

15. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression to...

16. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...FRET measurements and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation...

17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...

18. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...

19. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...editing in their livers, while non-injected and control gRNA injected mice had no detectable editing. Additionally...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...