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We narrowed to 821 results for: nin

Showing: 301 - 320 of 821 results

  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...HypaCas9, are great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species....critical enzymatic residues has been converted to an alanine (D10A or H840A), changing its double-strand cut...available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA, a reverse....   Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may carry a validated...expression systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression...

  2. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    Published
    Sept. 7, 2021, 1:15 p.m.
    ... shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with ...details about personal protective equipment to learning about how to use a tissue culture hood and other...on our Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists!...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...sj.emboj.7600385 McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing shRNA...shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing the plasmid backbone... and just needs to be taken into account when designing experiments.   {% module_block module "widget_...Kilikevicius, A., Meister, G., & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...3’ flaps containing the edited sequence anneal with each other, while the 5’ flaps containing the original...First, twin prime editing installs two sequences containing attB/P recombinase landing sites on opposite ...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome at this ...rat brain sections stained with Anti-GFAP. The staining patterns from recombinant antibodies produced ...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...been around since 1975 thanks to the Nobel prize winning work of Milstein and Köhler who developed hybridoma...by infecting bacteria for additional rounds of panning and scFv characterization.    Figure 2: ...by infecting bacteria for additional rounds of screening. Created with Biorender.com.   Some phage ...and VH sequences. Phage display also allows for screening scFv generated from the B cell antibody genes ...Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute to the disulfide...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...months. Jennifer and I spent about an hour that morning scrambling. Everything we had set up involved multiple...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...smooth filming in the future, but we were finally refining the process down to a place I could be happy with...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ....  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl (Ambystoma...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...involves the introduction of a transgene construct containing fluorescent proteins (XFP) of different colors...not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice...are that it does not use ubiquitous promoters, meaning that not all cell types can be labeled, and that...labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express the... a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...iBiology which hosts an amazing amount of useful, entertaining science lab, career, and policy content. Watch...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many...force it. You can always relate to scientists by explaining how your work fits in the bigger science picture...that this might sound condescending, consider explaining the term on a slide but not reading the explanation...practice. I always take a poll of scientists in training to see how many in my audiences have given a 50...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial host strain...The pCRT7 NT TOPO vector contains a mutant pSer-containing GFP that can be used as a positive control during... researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule that binds specifically...that wasn’t originally a serine (e.g. mutating a threonine residue to a phosphoserine) to control for effects...large proteins by Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...Off-target effects” a vague catch-all term for unintended consequences in an experiment. For CRISPR work...In other words, they can sometimes localize to unintended sites with sequence similarity to the on-target...on-target site. When a CRISPR-Cas system localizes to unintended sites and performs its programmed function there...not Homologous Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023...factor, etc.) this control is usually not very meaningful. Unfortunately, there really aren’t any effective...

  12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded DNA repair templates Short...electroporation methods using in mouse transgenics. Designing an Easi-CRISPR knock-in allele To design a knock-in...make the first two bases of the left homology arm guanine (i.e. GG); this design has been shown to increase..., you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The design for... can amplify the desired region using primers containing the homology arms on both sides and the T7 promoter...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ... aim of improving professional development and training in a globalized world where individuals can exchange...IMP 2014-15, University of Valencia): “At the beginning, I registered at IMP to have an opportunity to...throughout the entire year. In addition to their direct training role as mentors to Spanish mentees, mentors also...addition to a pre-program self-assessment survey defining expectations of the program and their baseline...resources, and entrepreneurial visions thereby opening new frontiers for young talent around the world...

  14. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...Crystal developed her management skills - training, learning from peers, and being receptive to those ...experiment. Chapter 6: Advice to scientists who are planning on working in industry. The importance of recognizing...our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens' AMA ...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its Fruit Is Sweet...types not within the vicinity of the injection. Examining serotypes 1, 8 and 9, not only do they undergo...transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course, one cannot...at the University of Manitoba. Her project is examining the use of DREADDs (Designer Receptors Exclusively...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...

  16. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...thresholds for validating CRISPR experiments, many are turning to next-generation sequencing options over older...configuration, produces many false negatives Combining sequencing techniques can ensure validated experiments...Editing Specificity” by Tycko et al. 2016 and “Defining and improving the genome-wide specificities of.... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities of...

  17. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...articles in less-prestigious journals have been gaining ground. A study issued by Google in October entitled...report also noted, papers detailing Nobel Prize-winning research typically weren’t among the most cited...impact.” As we look ahead to the end of a year and beginning of another, perhaps it’s worth reflecting on a...Other Novel Measures for Scientific Impact" from Opening Science - The Book Bollen J, et al. "A Principal...

  18. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...countries. We, like everyone around the world, are learning to navigate the changes brought about by the pandemic.... As always, we seek to understand our role in opening up science to help create new knowledge and research...opportunities. I have spent the last 9 months quietly learning about Addgene’s past and present, but the real...place in it. This period of careful reflection and planning will allow us to continue to serve the scientific...

  19. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...providing cloning grade DNA to you, we'd save you days of time so that you can begin your cloning experiment...this year were influenced by COVID-19 and our new cloning grade DNA service. We've had 11 blog posts on COVID... and industry. This year, we also launched the cloning grade DNA service. This service features 210 plasmids...

  20. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...was fortunate to see the event planning unfold from the very beginning. The board picked a theme (Bio-...DayCon DayCon and other similar events show how meaningful science outreach can be, both for scientists ...communication and organizational skills provide great training for a number of alternative careers in science...
Showing: 301 - 320 of 821 results