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Showing: 301 - 320 of 694 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...are being up- or downregulated in a system, detect post-translational modifications, quantify protein levels...immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications In addition to protein...abundance, westerns detect changes to a protein’s post-translational modifications such as phosphorylation...

  2. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...where you can thrive and do your best work. This post is focused on choosing a lab, but almost all of ...blunders, setting target up to fail, shifting goal posts without telling the target Warning signs while ...Learn about careers in science communication Read posts on mentoring for scientists Resources on Addgene.org...around and accessible for advice and input? Will a postdoc or lab manager be your actual mentor? Is there ...attending career events or being involved in a grad or postdoc association? Will you learn how to run a lab if...a way to grow and learn. Graduate students and postdocs in particular must care about this. The training... than one lab when you choose a grad school or postdoc lab. Having a comparison can be very helpful in...

  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded.... For a more detailed review, check out our blog post: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems...prior to irradiation with UV light (365nm) and that post-UV exposure it showed cleavage activity similar ... the Addgene Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon...

  4. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to me:...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale,... Keyence BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green ...phosphorylation is one of the most abundant forms of post-translational modifications in cells and research...see the detailed plasmid kit page or read our blog post: Quick, Versatile Plant Transgenesis with GreenGate...Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Addgene's First 10 Years Addgene’s Top 10 Blog Posts from 2014  ... in the presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...continue where the guide left off - namely, a blog post devoted to the other affinity reagents.  Affinity... antibody?  That is the question… or is it? This post isn’t meant to convert anyone to downsize their ...

  7. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... subscribe to our CRISPR blog content.   In this post we will cover: Miniature CRISPR-Cas systems SEND...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "... the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages Read more...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In...!” — Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016,...

  10. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...suggests changes in RC2H1 protein expression and post-transcriptional regulation of SM1v1-containing RNAs... by IRE binding proteins, IREB1 and IREB2, which post-transcriptionally regulate iron metabolism pathway...

  11. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in ...information, sign up to receive alerts for antibody posts from our blog!  Resources and References  Resources...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency...observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair...

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... an in-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review ...the other a crRNA array plus a GFP tag. Four weeks post-infection, there was strong expression of Cpf1 and...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement...cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...degradation of important cellular RNA molecules with post translational modifications. One of the most common...picked your perfect guide; now check out our blog posts on choosing the best Cas9 enzyme to pair with your...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...science career related blog posts Read our science communication blog posts Resources on Addgene.org ...widely. The way you communicate your science at a poster is going to vary depending on the conference you... in an academic lab setting, where student and postdoc turnover happens regularly and it behooves everyone... words like this when starting rotations or my postdoc would have simplified the first weeks or months...you discover during grad school or during your postdoc that you enjoy and have a propensity for cross ...

  17. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can...leverage this system in new, creative ways. Today’s post will focus on one such strategy--the FLEx switch...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...biological phenotype in a scalable way. This blog post will introduce you to this new technology, and guide...

  20. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades.... She then moved to France where she worked as a post-doctoral fellow on developmental biology at the ...Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene website Visit our molecular...
Showing: 301 - 320 of 694 results