...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...and engage with the public, and learn from the process of writing papers and proposals.  To get the most...

...to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant... methodologies drew me to learn more about the process that was used to create this important collection...

...axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected...way the brain connects across these regions, a process driven by neurons. By studying how neurons are ...

...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...output from the first process to the second process. For example, our bbduk process created trimmed FASTQ...pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines which command or script will...specifying the output of an initial process as the input to a subsequent process, we linked each step in our ...pipeline steps into processes and then connect the steps using the output from one process as input to the... Nextflow process.  Next, we specified the input and output channels for the initial process and tested...was clear: to build one process at a time and connect each subsequent process to the existing ones. Once...

...that numbers are not switched around or misread.  2. Track the container - It is just as important to ... be tracked easily at each step in the storage process. After the original sample is barcoded, the lab...stocks, prepped DNA ready for our quality control processes - all of these samples have the 2D barcodes mentioned...a rack of tubes is dropped at any point in the process, we know exactly which location each should go ...step in the receiving, transforming, and storage process, we ensure that the sample tubes that we use match...Addgene’s secret to success. It helps us quickly process samples accurately and in a way that can be easily...deposited samples along every step in the storage process - from deposited DNA to transformed cells and finally...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...the grant development and management process or the whole process, including grant readiness, funding ... that likelihood. We provide expertise on the processes needed to successfully implement, manage and close-out... funds available and the increasing regulatory process for grants has fostered the demand for experts ...position that required some involvement in the grants process and managing programs that were grant funded. I...and experience through the candidate application process, the candidate’s ability to write a grant application...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...powerful resources for investigating any biological process. High quality genome sequence A high-quality sequence...understanding the gene networks that regulate processes from development to metabolism to fertility. Several...mutations in genes affecting diverse biological processes—not just development—are available from strain...Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the zebrafish...and explain how signaling pathways control this process. Additional zebrafish applications and conclusions... providing unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow...

...unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

...IgM, IgD, and IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the...next to a different constant domain gene. This process is called isotype switching and results in the ...

...state-of-the-art methods of sex and gender analysis and 2) provides case studies with concrete examples of how...sex-based influences on physiologic or pathologic processes. Much progress has been made in this area in recent...

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their... become more actively involved in the learning process. As trainees become more comfortable with the open-ended...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...of students active participants in the research process – so if this sounds like an exciting opportunity...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...plants doesn’t always have to be a time-consuming process. While developing transgenic hairy root lines in... longer, a transient plant leaf transformation process could save the plant biologist some time… months...This method is commonly used in C. roseus, but processes specific to photosynthetic active tissues cannot... In the laboratory we can utilize this natural process by replacing the genes on the T-DNA region with...other aspects of the transient transformation procedure (Mortensen et al., 2019). Our method might provide...

...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

...system (by measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...

...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct... toxin which can affect a variety of cellular processes and an antitoxin that suppresses the toxin’s activity...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...!     Figure 1: Flowchart showing the process for using pooled lentiviral libraries. A) Amplification...of your pooled library during the amplification process. It’s far better to verify via NGS now than to ...want to consider spinfection. Spinfection is the process of transducing cells at a concentrated seeding ...

...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...need fewer resources for the entire translation process. For example, during high growth rate conditions...segregate with the cell pellet during purification procedures. Even if you produce a large amount of protein...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method... products that help to further streamline this process and/or stabilize RNA during tissue collection and...

...1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning Golden Gate cloning...enzyme recognition sites. As a result, the ligation process is close to 100% efficient. Another strength of...