We narrowed to 695 results for: prot

...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...about making your own competent cells and our protocols page to learn about bacterial transformation in...example, this plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green....plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find our molecular biology...biology reference here Find molecular biology protocols here ...

...right)est Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids...tool in neuroscience research. These fluorescent proteins light up in response to calcium, which is more...Aggarwal et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). Figure 2: OCaMP structure...various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND... OCaMP, including detailed characterization as protein or in neuronal cultures, zebrafish, and mouse brain...

...ability Experimental guidelines and applications Protein structure and properties Links to relevant resources...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...performing a viral delivery of Cas, the size of the protein would be helpful to know for viral packaging limitations..., RefSeq ID, etc.) and you will see a table of protein entry results. For the advanced search options,...from Adler et al.   You can also search by protein sequence with BLAST, using the homepage search ... them by other parameters, such as E-value for protein sequence searches. Additionally, the Phylogeny ...

... tumor microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function...Furthermore, tumor cells alter their cell-surface proteome to inhibit expression of co-stimulatory molecules...major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by CD8+...formation and metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb...Antibodies that block cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic...-L1 pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research...altering tumor cells and immune cells to knockout proteins involved in immune tolerance. Recently, China ...

...tracing with fluorescent proteins in nontransgenic and cre driver mice. Current protocols in neuroscience / ...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ... Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of opsin ...specificity. Viruses that only express the fluorescent proteins are used as a critical control. Depending on ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...plasmids! Find AAV resources! Find fluorescent protein resources!    ...

... Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can alter ... even between many cells (through gap junction proteins which connect the cytoplasm of two cells) can ...controlled by the opening and closing of ion channel proteins, they are not always easy to track at the transcriptional...University has used Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel... a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness and improved...J., et al. "Long-range neural and gap junction protein-mediated cues control polarity during planarian...

...Addgene to set up assays to see what molecules or proteins are involved in IL-36 release in keratinocytes...How GNB1 mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside...responses. For example, GPCRs and their downstream G protein partners can regulate ion channels in the brain...standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization, signalling...science education at McGill University.  These protein-receptor complexes consist of many parts that work... GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental...

...stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 Zhao, L., Koseki, S.R.T...or non-existent PAM requirement, and how these proteins are advantageous in today’s genome editing landscape...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...requirements. Luckily, there are some available.   Cas proteins that don’t utilize NGG   There are plenty of Cas9...developments below!) (Walton et. al, 2020). These Cas proteins have everything we know and love about Cas9, but...

...Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted Genome Editing (...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide interest...et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038...2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare (Preprint...Whether you plan to purify or image a recombinant protein, the FLAG tag has proven itself a good option for...

... RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end to protect them from...This editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU tracts...PE7 (right) offer two different strategies for protecting pegRNAs from degradation. Created with BioRender.com...prime editing with an endogenous small RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038...

...consist of a VSD fused to a single fluorescent protein (FP). This class of GEVI was the earliest developed...that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport, not ...viruses also have various serotypes – the surface proteins that confer affinity for a target – and this serotype...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them for targeting...Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the... Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

...application, like protein production. Titration of baculovirus can vary depending on the protocol used. Plaque... viruses, though they are a popular choice for protein production. The most commonly used cells in insect...is what makes insect cells a popular choice for protein production. AAV and lentiviruses can be produced...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...

...guidelines here. A detailed transformation protocol, as well as protocols for growing Arabidopsis are also available...transcriptional and translational dynamics of genes and proteins in living plants, to complementing mutant phenotypes...and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or...more than one construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium...your insertion in the absence of selection. For a protocol for antibiotic selection, check out Harrison et...

...able to come of age. The in vivo assembly cloning protocol In Vivo Assembly (IVA) cloning (Garcia-Nafria ... to be performed using a simple two-step, 2 hr protocol prior to transformation (Figure 1). As this approach...procedures are performed using the same three step protocol:  Fragment generation - Any linear DNA fragments...epitope tag can be moved to the N-terminus of a protein-coding sequence, or a mutation can be introduced...plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find our molecular biology...biology reference here Find molecular biology protocols here  ...

... of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry... graduate school. As a stable, easily purified protein with easily measured activity, MDH is particularly...structure, function, and interaction with other proteins.   It is important to note that research ...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),..., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings of the National...

...recognition sequences of an exogenous DNA-binding protein such as LexA into the target locus, which is the... DNA-binding molecule technologies such as TAL proteins and CRISPR became available, I was very excited...easily isolated for identification of associated proteins, RNAs, and other genomic regions. I believe that...analyze signal-induced nuclear translocation of proteins in 2004. In addition, we have recently developed...Addgene Alerts box. References Identification of Proteins Associated with an IFNgamma-Responsive Promoter...genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin...

...that the protocol is not approved for clinical use at this point, they hope that the protocol serves as...few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple,...has adapted natural RNase activity of the Cas13 protein to develop and optimize the method termed Specific...et al., 2018). Now, the Zhang lab has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The...endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/...

...have a natural preference to stay in dark areas (protected) as opposed to brightly illuminated ones (risky...environments by initially moving along a wall (protected) rather than being out in the open (risky). Taking...comprised of a brightly lit (risky) and a dark (protected) area. When measuring the time spent in a particular...derived neurotrophic factor (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and...Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons upon light stimulation. ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...there are many protein-based toxin-antitoxin systems. One of the most well known protein-based toxin-antitoxin...specific sequences in RNA resulting in a blockage of protein synthesis. The antitoxin MazE, which binds and ...inhibits MazF, is continually cleaved by the ClpAP protease so without continual production of MazE, the toxin...Toxin-antitoxin systems are not just restricted to the protein realm. Let’s take a look at a toxin-antitoxin system...additions to constructs that are used to drive high protein expression in vivo. Expression plasmids are often...

...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools that result from their...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding... fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal...the brain allows the squid to create a different protein. Did you know that most cephalopods die after they...