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Showing: 301 - 320 of 640 results
  1. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage more involvement...
  2. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working...
  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently...
  4. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent...
  5. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...
  6. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...disengagement. By tailoring my leadership approach to each person, I was able to create a more productive and cohesive...similar transitions can provide you with insights, lessons from their experiences, and practical advice for...
  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic...
  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...
  9. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...mesophilic systems are the psychrophilic enzymes.  The reason behind this isn’t really hard to guess. Think about...for all the Indian iGEM teams to interact with personnel from the industry and brainstorm with each other...
  10. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...started working in a new lab and I was the only person in the lab. When I saw a request for an Addgene...scientific conference, whether you are attending in person or monitoring that conference via social media....
  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies...
  12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...
  13. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield...
  14. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...
  15. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...
  16. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...
Showing: 301 - 320 of 640 results