...yearly planning meetings revolve around two simple worksheets, each of which is filled out separately by the...the trainee and PI before the meeting. These worksheets, summarized briefly below (and available of in...facilitate honest conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional...often give ourselves credit for what we have accomplished. Starting with a recap of accomplishments, from...

...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

...wanted to do when I grew up until I was all but finished my undergraduate degree (and I hope I’m not alone...towards a career in science communication, an established field of inquiry with which I was not yet familiar... a scientific background and bring with them established interests and areas of expertise. Perhaps the...strongest aspect of science communication, then, is its sheer versatility in the face of disparate spheres of ...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...

...available. With new fluorescent proteins being published every month, deciding on the best protein for ... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...common use (particularly not the most recently published ones), they can be a good starting point. In many...abundant proteins or marking compartments. I hope this sheds some light on multicolor imaging with fluorescent...

...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various serotypes available ...by the small molecule clozapine N-oxide (CNO) (Krashes et al., 2011). Viruses expressing pAAV-hSyn-DIO-hM4D...

...scientific communication needs. Going to present some unpublished results at a conference? Post your working manuscript...scientific community. Most preprints are eventually published in journals after formal peer review, with the... as the source or were deposited when the now-published article was a preprint. Depositing plasmids that...bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019) A proposal for the future of scientific...

...affinity: stronger binding antibodies will require harsher stripping to remove, so start with the weaker binding... membrane will remove some of your sample —and harsher stripping means more sample loss. If you are still...a recipe like the ones shared by Bio-Techne, ThermoFisher, or Millipore Sigma. We’ll share the basic composition...solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems offer...

... easily applied to multiple dishes, remember that handling multiple dishes will take longer and may increase...scaling up by either increasing the number of 10 cm2 dishes or increasing the vessel size. Opting for a larger... opt for simply increasing the number of 10 cm2 dishes. While this option is attractive because the same...

...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and...