We narrowed to 885 results for: ANT;
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TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....recipe. It’s unrealistic to plant, grow, and harvest grains every time you want to bake a cake. Instead, ...development happening at Addgene under the hood. A significant portion of our development may not be visible...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...
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Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated...different viral membranes, tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017) Table...its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA receptor-envelope system (Wickersham...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m.... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...retreat to the woods for months at a time, so take advantage of whatever time you have. If you’re like me, ...were, and you could find the original paper if you wanted to learn more. Another example: we made a schematic...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....Biddle, of Only Good Antibodies, who shared their antibody validation expertise. Antibody Validation for Flow... Caitlin Faulds, who introduced the very first antibody deposit (the Integrin collection) from the Institute...Institute for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who ... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....and spread over the second quadrant. The process continues until all quadrants are used, hopefully revealing...plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through...revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually successful...characterizing the microbes from the termite hindgut. Want to level up your or your class’s streak plate skills... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce in the...group developed Zebrabow as a system that takes advantage of the multicolor labeling possible from Brainbow...developed from the Brainbow 1.0-L construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP...fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods and can be labeled ...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation..., Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal Epithelia in a Mouse Model... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply...constrained. This collaborative approach eliminates redundant work, saving time and allowing precious research...DNA sequencing, viral vector preparations, and antibody production that labs may find increasingly difficult...services can be listed as direct costs from a CRO in grant applications, addressing the new funding structures...scientific community for the benefit of everyone. We Want to Hear from You We invite researchers to share ... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog PostPublishedJan. 21, 2016, 4:52 p.m....Dr. Koeris' reasons for wanting to develop bacteriophage for use as antibiotics 8:26 - 10:30 : How Dr. ...MIT) where he worked on understanding bacterial antibiotic resistance. During this time, Dr. Koeris and ...bacteriophage (viruses that infect bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded...36:53 - 38:00 : As an entrepreneur, is it more important to develop something that could succeed in the... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything ...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...validation data, sharing what works and, equally importantly, what doesn’t. Reports receive a DOI for citation... -
Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog PostPublishedApril 22, 2021, 1:15 p.m....been using Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...hanging out with Aavery in this Zoom video background. Want the purple bacteria video background with mascots...with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene’s viral...red COVID-19 background. If you’re predominantly a plasmid user, instead, don’t fret; we have... Did you get the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....system to developing an antibiotic that selectively kills only the bacteria you want it to, leaving all those...there's a pretty good reason for that. Lately, significant advances in the understanding and application...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....selection (ex: antibiotic selection and auxotrophy) and negative selection (ex: toxin-antitoxin systems and...posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators...Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to...us at [email protected]. Thank you again for a fantastic year and happy reading. Best wishes on your research... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....surprised at how many applicants ignore this in their cover letters. It is also important to express interest... Many job postings will result in hundreds of applicants the day they are posted. If your resume is not...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria... to highlight different skills and to get the important information in the right place on the page. 5)... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...Numerous applications have been developed to take advantage of the high fidelity of Taq and other thermostable...nucleotide sequences with high specificity and quantitative accuracy and even include profiling single nucleotide...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...different length, with products identifiable and quantifiable by capillary electrophoresis (CE) (see below...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....with the relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata...treatment can browse through screens performed in relevant cell lines or conditions. This increased ease ...enzyme system, sgRNA library and other details relevant to screen result interpretation. The analytical... Screen results are reported as the original quantitative gene-level scores provided by authors rather... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....features you may want to consider as you make your choice. The first and most important thing you need ... achieved with the proper selection marker or antibiotic resistance. Next, consider the expression level... or purify it – and you do not have a specific antibody against the protein available – you can always...be a more robust expression system and has its advantages; however, remember that you will need accessory... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m.... you may want to work on in your future career, so the research topic will be more important. In addition...selecting a lab with a PI who is a good mentor is important in both cases. As a PhD student you need to learn...scientists. The specific research area is not as important as choosing a supportive, positive role model.... opportunities to learn and practice successful grant writing, lab budgeting, equipment acquisition, public... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....share your AAV data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene...Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment conditions like drugs (Chemogenetics...designing your experiment, read our blog post on important considerations when using AAVs. Experimental results... we also take negative data, which is equally important to report. We recently published a paper in Frontiers... hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three Key Considerations... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein... Noda, Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m.... bacterial culture, only the plasmid with the antibiotic resistance gene and origin will replicate, and....7 kb recombined vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...(up to 24 hr), reducing the concentration of antibiotics (ie. 50 μg/mL Amp instead of 100 μg/mL Amp), ...MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid DNA without ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations.... mTFP1 is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi...excitation maximum 399 nm) because it does not significantly excite at 400 nm while EGFP does. Find mWasabi... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....your formal scientific communications, they are fantastic fodder for science communication. The trick is...something that sparks their interest and makes them want to know more. This could be by outright stating ...story. At this point you may be thinking “I don’t want to bury my results all the way at the bottom of ...other affected people, man/animal vs nature, anthropomorphized animals, or even seemingly unrelated stories...storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! ... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding RNA guides to...replicated in larger studies, it will represent a key advantage over Cas9, for which gRNA sequence can greatly...aa depending on the species). This feature is advantageous if you’re working with small capacity viral ...via indel formation, this property could be an advantage. However, repair after base excision would alter... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m.... laptops and some server space. But what if you want to be an entrepreneur in the health sciences? Is...The composition of the management team is more important than the product itself -- investors would rather...majority of these meetings acted as a platform for significant company advancement. Be relentless The world ...against you. As I mentioned, you start at a disadvantage in the health science space if you don’t have... to it. First impressions are everything and you want to leave people remembering your potential as an... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....used to negotiate individual MTAs every time they wanted to share materials, and the process was time-consuming...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...process. And you do have options for the terms you want to share your biological materials under! While ...for both research and commercial purposes. It’s important to note that it’s the depositing institute that... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m.... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...hurdle in front of publication when all they really want to do is get the paper off their desk and move on...only a partial solution. How do we get where we want to be? The part that is still missing is a clear...management plans and for evidence of sharing for grant renewal—but there is clearly room to do more. If.... Sounds like a win for everyone. If we agree we want to get there, that’s half the battle. Many thanks... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....community, so it is extremely important for us as a company to constantly update our processes and get ...for Fragmentation System and this is the most important part of this newly released prep kit. In their...itself. This was great because it forced workshop participants from different biotech companies to interact...Therapeutics, a biotechnology company that uses neoantigen biology to treat cancer. My partner for the day... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....growing the cells on solid plates, where each transformant forms its own colony, uninfluenced by the growth... from both. These products are then purified, quantified, and sent for sequencing. Analyzing your sequencing...corresponding sequencing quality. Generally, you want to have 100x-1000x coverage of your library: in ...very similar to each other - that’s exactly what we want! At the end of the day, it’s up to you to decide...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....connections Here are a few things you probably don’t want to do: Don’t connect via LinkedIn with strangers... for favors. Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...4,700 LinkedIn connections. I mention this because I want it to be clear that it is completely impossible ...interest in you. Network with the people that you want to be with. Then, the following up will be easy.... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting...basal fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large ...-WPRE 105322 AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262 AAV1 pAAV-hSynapsin1-axon-GCaMP6s...Name 105322 AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...