We narrowed to 842 results for: Eras

...and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist from Bloomberg news. Given ...who shared first authorship on their seminal, cooperative paper in Science.  Interestingly, this project...finding they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling..., open academic exploration in this area will accelerate the launch of clinical applications. During ...

... brand new Europe office located in London and operated in collaboration with LGC Standards. And now you... VII. During my years in research, I developed several new plasmids, including: plasmids to study the ...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These...sharing those ideas with others. This love of interacting with people and talking about science makes her...

...behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA...body. Administering the drug once can result in several hours of neuronal excitation or inhibition. This...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects... by Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...

...of time, especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...Plasmid DNA can be stable at 4°C or even room temperature for a short period, and there are indications...

...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...washing. Nonetheless, this tag can be purified with several different resin types and is a fairly small tag...and other qualities of each tag class, they are generally best suited for their primary application. Ready...

...cell line collections websites and catalogues Literature searches utilizing Google Scholar alerts and ...feature to know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa ...information on the known features and relevant literature, or to decide which line is best for your experiments...Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding errors in the available...

...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...lights (Baier et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit...circuit involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...

...receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However, the R206H mutation...including Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes ...interferes with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical...

...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...studying to earn a PhD. The society encourages interactions between undergraduates and PhD students so that...develop a range of talks for both students and the general public, career events, and networking meetups. ...

...included in organizational decision-making. It is imperative that employees are thanked for their work by ...but it is worth it. This requires a focus on deliverables. It does not mean a manager must allow his team...enough of a jerk to make the rest of the team miserable.   A good manager keeps these factors in mind...decisions, but that should not be the common mode of operating.  Managing scientists I should add a few special...

... concept that these clustered repeat sequences operated as a safeguard against bacteriophages did not ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...proteins then express and process the CRISPR loci to generate RNA, which guide a Cas nuclease to the specified...learned to circumvent the CRISPR/Cas defense by generating a single point mutation in the PAM sequence, ...

...publications—the pressure to produce is the same You get to interact with scientists just like you What is different...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

...study which can be accessed here at bioRxiv. AAV2 interacts with the packaging cell's cell membrane When packaging...blocked following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose...    Despite observing a positive effect on our overall yield, we were unable to demonstrate that these...-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. PubMed Central...

...developed in David Liu’s lab in 2019, has added considerably more precision and flexibility to the CRISPR... repair favoring the edited strand. The first iteration of prime editor simply fused the wild-type Moloney...pairs for prime editing. Since PAM sites occur on average every ~8 base pairs on either DNA strand, many ...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...system PE2, which had prime editing efficiencies on average 2.3- to 5.1-fold (though up to 45-fold) higher ...

...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...from the manufacturer and/or in the published literature. Note that the antibody being published doesn... used in combination with other approaches. If several cell lines express the target at different levels...contains information about the labeling pattern of several cell lines using these two antibodies. This is ...

...residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The iodine-modifications...cells.  He et al also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed... red fluorescent photosensitizer protein that generates ROS when illuminated with green light. They found...inactivation/cell ablation while having limited collateral damage to bystander cells. Are you ready to use...

...expression, localization, and protein-protein interactions. Beyond these common applications, researchers...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation... upon exposure to ultraviolet light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions...

...of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories ...each well of a 96-well plate. Then, samples from several plates are pooled together to create a single library...harder and harder to obtain balanced and sufficient coverage of all samples. The root of the normalization ...important for getting adequate sequencing depth and coverage in order to, for example, assemble hundreds of...

...Integration. Molecular therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address...be to prepare them for the arduous process of generating viral particles. Limit tube transfer - the fewer...

...visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools using CRISPR...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ... tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

...plasmid: name, description, how the plasmid was generated, validated sequence information, and more. The...persistent identification of research reagents, several journals or organizations now recommend or require...authors to use specific PID formats. For example, several journals like eLife, Nature, and Cell use  Research...copy and paste the text attributing the PI who generated the plasmid, followed by the PID(s) of your choice...

...behind Gram’s crystal violet stain and the ion interactions that cause the stain retention in gram positive...When the negatively charged iodine is added, it interacts with the CV+, creating a complex within the peptidoglycan...trapping the large dye complex created by the interaction of CV+ and I- in the thick multilayered membrane...can be used in medical settings to determine the general type of bacteria present in an infection. This ...

...depending on the primer sequence and the NEB polymerase used. Overall, while much of the information in the ...helpful, but does make it easy to skim the manual. Overall, it’s a great tool for someone starting cell culture... it had a codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or ...

...distances. For instance, how much force can be generated by conformational changes in proteins? How can...can be addressed using R bodies: simple, force-generating protein machines that can extend and retract ... might be able to endow cells with new ways to interact mechanically with their environment.   Jessica...

...to interview, part of the interview process was several short talks by current faculty members and it just...it looks good, that’s a high that can last for several days.” Working with CRISPR Recently Doench has...since” and that he’s “very much in line with the overall mission of making science easier and more transparent...s] outreach scientists do to comb through the literature, to go and talk to the people who actually have...

...study microbiome interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice...within the biological sciences, there’s plenty of interaction between neuroscience and other fields. For instance...us produce new educational content on the blog. Overall, there are many exciting things going on in the...

...expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because ...technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using Cas12a’s ...similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple genes within...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

...post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards...smaller than 10uL. You can leave them at room temperature when you’re working with them, but avoid freeze-thaw...okay, Blugene!    Buffers  You know what temperature to store your antibodies at, but it also matters...commercial kits.  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots...

...content - the useful information on scientific literature, technical skills, and experiences from thousands... Nearly everything we write entails a primary literature search to collect up to date information on the...review process led to quick dives back into the literature to make sure absolutely everything was correct... typos, and hit publish. Making content is an iterative process. We are constantly reviewing the resources...

...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate...drug-containing media in which only cells which can tolerate the drug survive and everything else dies off....though. A selection can take the form of varied temperature, nutrient availability, drugs, and much more....