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  1. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ..., and almost all universities and institutions require them for material transfers. When Addgene entered...and emails it straight to you, or we can send it directly to your institution’s MTA coordinator. Organizations...

  3. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled by a neuron...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes ...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...

  6. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  7. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...of newspapers. Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist...The other thing photography has shown to me - it requires an awful amount of skill,  time for which most...

  8. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and file ... to the necessary time and resource investment required to change the status quo. But with recent improvements...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

  10. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed for one dCas9...fluorescently labeled dCas9 isoforms are efficiently directed to the proper target sequence. They were also ...

  14. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...companies have the potential to make a huge impact directly on waste, but also on culture. Encourage your ...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...

  15. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...These apurinic sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations...concentration. Alternatively, if the priority is to directly use your DNA preparation in any downstream application...

  16. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ..., and, as mentioned earlier, how frequently the entire group will meet. You and your collaborators should...with everyone involved can speed up the future directions of the project. The state of research is constantly...

  18. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...

  19. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...gene The rep gene encodes four proteins that are required for viral genome replication and packaging: Rep78...

  20. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...2 components: 1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon...Beauty transposase as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is...

  22. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop buffer...commercial boxes were small enough to minimize the required area and buffer volume.  So, I designed my own...

  24. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

  25. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...and control elements. Development of this tool required creating a database of common features, and devising...Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity when detecting a...

  26. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...down viral genomes containing only the sequences required for viral replication and transcription as well...binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA...

  27. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

  28. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures...
Showing: 481 - 510 of 758 results