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We narrowed to 920 results for: Gene

Showing: 641 - 680 of 920 results

  1. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h_3xC2...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...specific with a small and minimally-disruptive genetic tag on the protein of interest. Table 1: Summary...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating Choices...

  3. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual... all drive Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world...distributed plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! ...Cambridge, MA 02139 Have a question? Email: help@addgene.org Call: +1 (617) 225-9000         ...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...create and use a standard curve and provide some general considerations for scientists planning to use them...for the known concentrations (standard curve), generating an equation that allows you to determine the ... triplicate. The closeness of the data points generated by replicate values provides useful information.... With the help of graphing software, you then generate a trendline for the data. The appropriate trendline...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...

  5. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...

  6. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...develop a range of talks for both students and the general public, career events, and networking meetups. ...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you...molecule into the cell. The fluorescence that is generated allows for qualitative and quantitative measurement...placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the... we’ll take a deeper dive into the content of Addgene’s aptamer soup by exploring the development and ...and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ...changes in intracellular viral rna." Journal of General Virology 98.5 (2017): 992-1003. PubMed PMID: 28555544...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization...

  9. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...infiltration into a cell. The twist is that the typical genetic cargo — the viral genome — is swapped, at least... viral genome has been swapped for alternative genetic cargo, they can’t reproduce more functional viruses...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people...Institute for Neurobiology has been working on genetically encoded indicators of calcium and other small...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck... of the standard organisms for which extensive genetic tools are available. While he recognizes that some...PubMed) Lindenburg et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. ...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ... pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics continues to be a useful method for manipulating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...serotype testing Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (55645-...55645-AAV5)  Additional resources on the Addgene blog Read all of our viral vectors blog posts Find tips...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ...

  13. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...new ways of understanding how the body works, generating new therapeutics, and sometimes formulating new...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Initial positive results and the excitement they generate can, however, bias us. We’ve all heard stories...

  14. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... on the Addgene blog An Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a ... systems that measure biological phenomena by generating an output which is directly proportionate to ...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte... (fluorescence, pH, etc.). The transducer will generate a numerical value which represents the amount ...: Almost all of the biosensors discussed are genetically encoded, and luckily, we have pre-designed plasmids...disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn on, change.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

  15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...exhibit enhanced site accessibility In order to generate a genomic alteration at a specific location, it... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with improved ...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

  16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...are tumor microenvironments made of?  A tumor’s genesis is divided into 3 phases: initiation, progression...that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes...formation by inhibiting cell growth, invasion, and angiogenesis (Allinen et al., 2004). In fact, Sanford H. ...influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists began ...Fernando Schmitt. "Microenvironment in breast tumorigenesis: Friend or foe?." Histology and histopathology...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

  17. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...developed a dual virus system using both optogenetics and chemogenetics to study the activity of parvalbumin...in the lab. He has recently developed a third-generation intensiometric glutamate sensing fluorescent ...catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...withdrawal before they occur.  First, she used a genetic method to tag neurons activated by opioid withdrawal...light activates the neurons (via oChIEF) and a chemogenetic agonist inhibits the neuron (via hM4D). She ...postdoc at Harvard University where she engineers genetically encoded voltage indicators. Tian developed a ...optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator which had improved ...

  18. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...and learn more about Addgene’s viral service. Additional resources on the Addgene blog Download our Viral...service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed... in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral...applications, and some new resources available from Addgene. There’s a lot to talk about! And by the way, there...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...

  19. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...mouse inner ear. Fourth-generation cytosine base editors The fourth-generation base editors, BE4, reduce...activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope and efficiency...18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1,...these three improvements represent the fourth generation of base editors. Compared to BE3, BE4 offers ... phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which edits ~590-fold faster...., 2020). This is important because ABEs have generally been slow, meaning that the Cas9 domain often ... N. M. (2023). Improved cytosine base editors generated from TadA variants. Nature Biotechnology, 41(5...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing...towards researching neurodegenerative diseases.   Additional resources on the Addgene blog Check out all...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

  21. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...488nm blue light. The T203H mutation in PA-GFP generates a chromophore population that is more neutral,...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't...endogenous protein structure and function. By generating monomeric PA-FPs, there is less chance that the...induce though their continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists...PubMed PMID:18724362. 8.Stiel, Andre C., et al. "Generation of monomeric reversibly switchable red fluorescent...PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal...

  22. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

  23. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...introduction, we broke down the method into six general steps and outlined the considerations to be made...fixation*. Organic Solvents Organic solvents, generally acetone, methanol, or ethanol, work by dehydrating...to use your specific antibody for IF, look for general IF protocols for your sample type. Starting with...cross-linking fixation; they require an additional step generally referred to as Antigen Retrieval. I chose not ...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

  24. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make your...have integrations with a broad range of relevant generic and science-specific tools. In addition, it should...repositories Over the past decade or so a number of general purpose repositories have been launched and are... associated with publications into one of five general purpose open source data repositories: Dataverse...one and preferably more than one of these five general purpose repositories. RSpace has been integrated...@rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook...your work more accessible by depositing at Addgene ...

  25. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts...can be created in many different ways, but the general premise is that an aptamer in its unbound state...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...

  26. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene. gTBE...editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE...this promising start, the team used rational mutagenesis to produce the adenine transversion editor AYBEv3...and ABEs, they could be useful for saturation mutagenesis, and they provide a strong proof of concept for...empty space. Over several rounds of rational mutagenesis, the team progressed to gGBEv6.3, which was capable...s41467-024-50012-w Additional Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors...about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids for base editing Get ...

  27. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...publication. Who is to say that the initial data-generating lab has efficiently trawled their own data? A...much time they had. It could be argued that data generators may, in light of the policy, hold onto data longer... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating...amounts of storage. For example, mouse behavioral geneticists may track the motion of multiple animals on different... different occasions via video, generating huge amounts of data. When published, this data is usually ...as SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload...just a single projection of it, could allow idea generation and extension, based on existing, published data...

  28. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...science communication Resources on Addgene.org Learn how Addgene engages with the community ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...findings, and experiments as articles written for general audiences. So far, we are beginning to make an ...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

  29. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

  30. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...communicating science to the general public and training the next generation of science communicators. In...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... is a source of scientific information for the general public as well as a platform for scientists to ...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

  31. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister... in education, government, and civilization in general (1). Science and politics? Science is an apolitical...as evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link... human consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research...mostly due to human activity while much less of the general public agrees (5, 6). Many of the new administration...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...relatable (17, 20). This is an effort to show the general public that science is done by people like them...

  32. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...in freely moving mice Record DA dynamics of optogenetically activated and inhibited neurons in live mice...neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396 (2018): eaat4422... PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor enables rapid and ...PMCID: PMC6092020. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  33. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  34. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...own research project.  3. Do you prefer being a generalist? Working on a variety of projects and in different...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

  35. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics continues ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  36. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

  37. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters...to issues with tissue autofluorescence, cell heterogeneity, and/or cell position changes (To et al., 2016...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

  38. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...exciting revelation. Science in any field can generally be applied to improve our lives and solve global...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...also found that it is more common among first generation students, women, BIPOC, and other minoritized...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career... the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...

  39. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...where should you begin? Have no fear, Addgene is here to help!  General Design Figure 1: Two proteins...the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation...proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia Kenyon mRuby4 mRuby4(1-10), pairs...

  40. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...
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