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We narrowed to 830 results for: Eras

Showing: 321 - 340 of 830 results

  1. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ... the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting ...the next generation of scientists. The connection between mentors and Singletons can accelerate the networking...meet someone new, as they’d been introduced to several people by their mentor, as well as their bin-mates...recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons better...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

  2. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...opportunity to discover and evolve your transferable skills. Transferable skills are the non-technical skills... 5 o’clock. You’re going to have to talk and interact with...non-scientists! Maybe you’ve just spent... Wu in graphic design. You could dread these interactions, but you could also embrace them. Talking about...production, I didn’t really start to recognize my transferable (or “soft”) skills until I was out of the lab...lab to think about these--get started with our Transferable Skills Guide.     Over the last decade, my... blog Visit our careers topic page Read our transferable skills guide Read blog posts about career readiness...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...means they can be used with or as fluorophores in several different ways. Researchers have used oligonucleotides...can be created in many different ways, but the general premise is that an aptamer in its unbound state...cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium...fluorophores, including: Environments with higher temperatures, since proteins can denature while aptamers ...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

  4. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...and some considerations when using the method. ELISA basics All ELISAs follow the same overall process....simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background... the capture reagent to prevent non-specific interactions. Next comes the binding step where the target...Typically, the reporter is an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP)... of conjugated antibodies   Additional considerations No matter what ELISA method you choose, it ...

  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...initiation proteins to start their own replication. In general, these plasmids tend to be low copy number. Conversely...at the low-affinity sites, the proteins work cooperatively to separate nearby AT-rich regions of DNA. In...are occupied by ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.com... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...controlling the initiation of replication. Once the average copy number for a given plasmid has been reached...called iterons located near the ori and directly interacts with DnaA, stabilizing its binding and contributing...negatively regulate its own expression and thereby the overall replication of pSC101 (del Solar et al., 1998)....

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, that overcome...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called...specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production process...creation of CAR-T cells directly within the body. Overall, DIRECTED can increase on-target delivery with ... offers a promising approach for experimental therapies and medical applications.  Find DIRECTED plasmids...Lacy Proximity labeling can identify protein interactions or map a local proteome, but some applications...transcriptional history of a cell. Lin et al. generated a fusion protein called iPAK4 that assembles into...

  7. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...India at room temperature. Partnering with our distributor allowed us to ensure that temperature-sensitive...transit for a certain number of days and tracking average transit time for each courier we contracted with...destination in a timely manner and at the correct temperature. To date, we have partnered with six distributors...country. ‌For example, when we began offering temperature-sensitive materials, we noticed a large percentage...preps and libraries were kept at the appropriate temperature for the duration of their journey. Figure 2... backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we ...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...your viral vector, you use the same protocol for generating lentivirus, but you use a different envelope ...terminals and can’t be used for neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein...Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116...Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb...transport and access to the nervous system after peripheral delivery. Human Molecular Genetics 10:2109–2121...envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes...

  9. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...process to reduce viral order fulfilment from an average of 40 minutes to 10 minutes. While users can’t ...result in less than optimal user experience and the overall soundness of the software design. Each of us brings...called Scrum. The Scrum framework is based on an iterative and collaborative approach to development. Scrum...capacity to finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily...allows for life to happen without impacting the overall work. Each of our sprints is themed and the stories... being maintained (i.e. hasn’t been updated in several years) or they make significant changes that make...that helps our users focus on the fun stuff, accelerating research and discovery. We work with transparency...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... slim chance of generating indels with base editors, keep in mind that the generation of indel sometimes...minimizes the second outcome and increases the generation of the desired T-A base pair. Figure 1...inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair...simultaneously.     These binary vectors could be used to generate stable genome edited plants via Agrobacterium-...transformation. Cytosine base editors for plant There are several CBEs available for plant systems. Target-AID based... transient assay. For stable transformation, regenerated plants may be subjected to genotyping. If successful...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...molecule into the cell. The fluorescence that is generated allows for qualitative and quantitative measurement...contact with its RNA aptamer. This increased interaction between Corn and its small molecule makes Corn...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...changes in intracellular viral rna." Journal of General Virology 98.5 (2017): 992-1003. PubMed PMID: 28555544...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...of organelles and vesicles, and more. Although several strategies are available to disrupt microtubules...extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment optogenetic ...recently developed a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et...express the other separately, then use blue light to generate CRY2-CIB1 oligomers that trap the fusion protein..., and 100 times the activity of the original, generated using directed evolution (Tei et al., 2023).  ...license.   Just make sure you don’t overdo it! The moderately-enhanced 10x and 30x SuperPLDs avoid dark-state...Krishnan, N., Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., &...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously...experiments. shRNA plasmids typically use RNA polymerase III promoters, which transcribe small RNAs. The..., shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is...plasmids One of the most common uses of shRNAs is to generate stable knockout or knockdown cell lines. In fact...collections/mammalianrnai/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...(2004). MicroRNA genes are transcribed by RNA polymerase II. The EMBO Journal, 23(20), 4051–4060. https...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...these techniques are cumbersome, often requiring several rounds of selection to isolate plants with the ...organisms like Arabidopsis and Medicago truncatula and several crop species including potato, corn, tomato, wheat... plant cells.   This blog post will present a general overview of plant genome engineering using CRISPR... repress target genes in plants using the same general experimental design principles developed in other...systems, multiple gRNAs can be expressed to modify several genomic loci at once (get more information on multiplexing...sequence to enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created... the plasmids described above, Addgene carries several useful CRISPR toolkits for creating plant expression...

  15. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...to three) working in your chosen field. This cooperative education experience (co-op) is what has brought...find out that the two hours were split up between several Addgene employees, who all made the daunting task...old) employees with information on how Addgene operates as a team and shows how they fit into the system... on projects there. At this point, I have seen several lab processes and am currently working on projects...are scheduled to take place and employees are generally expected to be available. In addition to these...student at Northwestern University. She is one of several students completing their co-op at Addgene. This...

  16. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution...high-quality antibodies and reagents that you know work…preferably before you start your experiment. (And you’re...the IBEX Knowledge-Base, seeking to create an iterative process that is constantly becoming better, with...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative immunolabeling and chemical bleaching method for...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

  17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...overlapping or nested on a register, then the operation of one recombinase on the register could affect...affect the way downstream recombinases interact with the register- either by re-orienting their recognition...amplified by a particular primer pair. Then, by leveraging qPCR data from multiple primer pairs, one can...designed to have 20-30 nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts...not – the state machine design space is highly degenerate and often times there are multiple register designs...serine recombinases have been described in the literature (5, 6) and may be substituted for one another...predictability of RSMs and recombinase-based circuits in general would benefit from a more comprehensive characterization...

  18. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...fluoresce; when present, calcium induces CaM/RS20 interaction, thereby changing the FP’s structural organization...M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer between the fluorescent..., and voltage across membranes. These sensors generally follow the same pattern described above - a sensing...withstand a particular solute concentration or temperature? For further advice, see our posts on how to ... Plate-based assays (plate reader) Detect the average amount of fluorescence across a population of cells...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...the relevant literature to understand where the field is today? Have you talked to veterans in the lab ...-to-date with the literature of a couple of well-known journals that discuss general science in the area... timeline? A typical research project can take several years to complete and has multiple benchmarks. ...before even touching a pipetteman. Take the time to generate new ideas and think about them critically before...

  20. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...looking to determine how Protein A and Protein B interact. You read extensive research on the two proteins... both targets in your specimen. You scour the literature and find an ideal antibody against Protein A ...reduce immunogenicity and thereby enhance the therapeutic potential of antibody medicines. In this process...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...utility of recombinant antibody tools, the resulting chimera may have different binding properties (Morelock...needs to be addressed when multiplexing. It is imperative that you use narrowly selective isotype-specific...
Showing: 321 - 340 of 830 results