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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 566 results for: FIE

Showing: 321 - 340 of 566 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... grad school, you searched for and eventually identified a postdoctoral mentor. A good mentor typically...yourself to and interact with the members of your field, and present your research. Face to face meetings...

  2. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...Lentivirus Production and Transduction Protocols   Once purified, you must titrate your viral particles to determine...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use the same tag...649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with different dyes. b)...

  4. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...sets. Significance is a numerical value which quantifies the likelihood that a result is due to chance...Benjamini uses a complementary approach which identifies a p value cut off. This method entails arranging...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...then you expect that the bulk of the peptides identified to be from the target protein. This approach ...the majority of the protein in the sample was identified as your target protein, allowing you to proceed...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...the key components of transposons, how they are classified, and how they are used in the lab. What is a ...Figure 2: Overview of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea... Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...cells (9). PA-FPs have helped to revolutionize the field of super resolution microscopy. PA-FPs, especially...reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6...

  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... use antibodies for experiments in a variety of fields including neuroscience and immunology. They can... the antibody using a low-pH buffer. Then, the purified protein can be used in experiments to compare ...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...introduce. We use Ultramer oligo from IDT (non-PAGE purified oligo is fine based on new data). For general ...homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes (>100 bp insertions...

  11. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...have some exciting news for you too! The CRISPR field is always changing so we’ve been busy updating our...

  12. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ... learn about new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating many of these...

  14. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Biology One of the best ways to learn about a new field is simply to know where to look and how to start...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...biological scientists from a variety of different fields. Exposure - With over 100,000 views each month,...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Lab - Control neuronal activity with a magnetic field. Listen to Our Segment on Magneto 2.0 Hot Plasmid...

  18. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...introducing science communication as a research field. And there will even be a few tips for scientific...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week spent in...
Showing: 321 - 340 of 566 results