We narrowed to 354 results for: HRE

1. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to the use of antibodies to...

2. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   Published

   Oct. 5, 2021, 1:15 p.m.

   ...domain. They can also be used in crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova...

3. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ...10 weeks. Adult mice reproduce as often as every three weeks so scientists can simultaneously observe several...

4. Transferable Skills Guide: Time Management

   Type

   Blog Post

   Published

   Dec. 5, 2017, 1:52 p.m.

   ...processes, I’ve broken down time management into three conceptual chunks: prioritization, planning, and...

5. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...and short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV,...

6. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ... insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts...

7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ..., Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable...

8. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...technological innovations. Let’s take a look at three historical techniques: southern blotting, restriction...

9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ... Essentially, the immune system doesn’t see any threat to which it should respond. Latently infected cells...

10. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    Published

    June 27, 2023, 1:15 p.m.

    ...Optogenetic tools Types of control We will discuss three types of tools: light-gated ion channels, optogenetic...

11. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...Whether you’re just getting started with two or three targets or you’re pushing the limits like Addgene...

12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...Quadros et al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with...

13. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...particularly useful when the gene being expressed has threshold effects, such as with signaling molecules or transcription...

14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...

15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    .... A typical RNAi experiment can be divided into three fundamental steps: creation of the RNAi trigger...

16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...should not have been changed for at least two or three days before sampling (Uphoff and Drexler, 2011)....

17. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...you are aiming to achieve. In general, these are three core goals of antibody validation:   Confirm reactivity...

18. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...2010). As the field moves toward standardized thresholds for validating CRISPR experiments, many are turning...

19. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both ...

20. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ... single, thin plane within a relatively thicker three dimensional sample (see Figure 1); similar to an...