We narrowed to 370 results for: Numb

1. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ....  PE7 shows enhanced editing efficiencies in a number of cell types at different genomic loci — in some...

2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... bacteria in favorable conditions double their numbers rapidly. The knowledge the Singletons gained from...

3. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...base editor platforms. Hua et al. constructed a number of plant base editors replacing the SpCas9 with...

4. How to Write a Scientific Review Article

   Type

   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ... thing I did was stare in horror at the limited number of hits. So, here, I will share my process with...

5. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...fits your experiment. This choice will depend on a number of factors, like your organism, desired expression...

6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...cells in real time. Previously, we have described a number of practical considerations that should be taken...

7. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...expression strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed...

8. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ... will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons) and the...

9. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria...

10. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies...

11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...addition, it increases avidity because it doubles the number of binding sites per unit.  There are several ways...

12. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...ideal model organism: quick development, a large number of offspring (produces many seeds), self fertilizing...

13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ...delivery of a plasmid. RNAi is an umbrella term for a number of different applications that involve different...

14. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ..., but data is not going change depending on the number of people supporting it.  3) Linking scientists...

15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their...

16. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ... for each homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...

17. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...versions have been developed, which differ in the number, stability, and brightness of the fluorescent proteins...

18. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...by the top strand. Both produced about the same number of positive clones. See Supplementary Note 1 in...

19. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ... methods for studying protein phosphorylation A number of classic methods exist for this, the most common...

20. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...in situ hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by...