We narrowed to 362 results for: Numb
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TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m.... will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons) and the...
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CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m.... for each homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....ideal model organism: quick development, a large number of offspring (produces many seeds), self fertilizing... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....versions have been developed, which differ in the number, stability, and brightness of the fluorescent proteins... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....addition, it increases avidity because it doubles the number of binding sites per unit. There are several ways... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....delivery of a plasmid. RNAi is an umbrella term for a number of different applications that involve different... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m...., but data is not going change depending on the number of people supporting it. 3) Linking scientists... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....by the top strand. Both produced about the same number of positive clones. See Supplementary Note 1 in... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m.... methods for studying protein phosphorylation A number of classic methods exist for this, the most common... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....in situ hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....excited. This approach may also be applicable to a number of rare genetic diseases caused by splicing defects... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....problem is that the discussions usually focus on the numbers, rather than the necessary resources. This is why... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....is expressed in the hippocampus, or counting the number of puncta in each cell. When making figures for... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.... repressible binary systems. In recent years, a number of more complex systems have also been developed... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....prediction is ever perfect, but this can decrease the number of guides one needs to test in the lab. Importantly... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics began as... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....treatment. Westerns are also a useful readout for a number of cellular assays. They can determine protein ...