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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 553 results for: PPL

Showing: 321 - 340 of 553 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...sequencing is laborious, time consuming and cannot be applied to heterogenous populations (Bell et al. 2014)....Genotyping Options Technique Detection Limit Application Advantages Disadvantages GUIDE-Seq 0.1% Cell-based...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct comparative...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost... unedited strand (Chen et al., 2021). The team applied this approach to both the PE2 and PE3 systems, ...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...published antibodies, check the manuscript and supplementary figures for validation data, or follow the references...it includes validation data relevant to your application — and double-check that lot number while you’...

  5. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

  6. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  8. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  10. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

  12. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...offers options of between 14% - 100% electricity supplied from renewable sources and we have chosen to participate...

  14. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... spent a year hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They ...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...
Showing: 321 - 340 of 553 results