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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: REV

Showing: 321 - 340 of 610 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...large field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features...

  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... vector can still occur even in the case of no previous immunity existing for that adenoviral vector (...(2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...more accessible way to image biological features previously only visible via techniques like super-resolution... disciplines now available. In this post, we’ll review the inspiration behind ExM, how it works, and some...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...large number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many... to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman in ...

  11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...require a PAM, and RNA targeting is potentially reversible since there is no genomic edit. Some Cas13 enzymes...KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech...RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...conversion by engineered glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143...

  19. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...the questions.  After the code is written, it is reviewed by at least one other developer. This is another...point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into the mind of...
Showing: 321 - 340 of 610 results