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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: Rest

Showing: 321 - 340 of 644 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%) and laborious.... Figure 1: In the background are 504 restriction digests of PCR products used to identify a single...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

  2. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not an excuse. Every...someone else on that team is putting their own self-interest ahead of the team’s goals, then that team is destined...other person really is acting against the team’s interests or not; once that seed of distrust is planted,...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...to a fluorophore. When paired with a protein of interest, these tools provide an easier means to identify...of cellular components, typically a protein of interest. Fluorescent dyes and ligands vary in their chemical...fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the...plasmids that express the reprogramming factors of interest. The advantages of this method are that it’s relatively...KLF4 LIN28 Check out these plasmids if you’re interested in Tet-inducible lentiviral expression of OSKM...

  7. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as...genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe University of Agriculture...

  8. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...move from microbiology to law? How did you get interested in pursuing law? BN: Life science was always ... general counsel at a small start-up is very interesting. It’s very varied. There’s IP work to do, general...

  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...and country. This information can be of personal interest to the depositor, but it can also be valuable ...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...

  11. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection can look at... selecting an antibody! We encourage everyone interested in the Addgene antibody collection to use and...

  12. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...Even that grim estimate might prove to be an underestimate. Given all the excitement and speculation, we...ongoing outbreak from the CDC and WHO. - For anyone interested in EBOV’s past, present, and future, don’t miss...

  13. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...commercial kits you will adopt for the extraction. Restriction enzyme based approaches can require several hundreds...cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection grade...

  14. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...Citing Article" - such as cloning their gene of interest into a previously deposited vector - then you'...locate other papers that have used your plasmid of interest, which may help you identify relevant protocols...

  15. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...trainees at all. For grad students and postdocs interested in taking matters into their own hands, there...I am happy to work with any group of trainees interested in designing career exploration programs or peer...

  16. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... from your sequencing runs.   When you run a restriction digest on a gel you always include proper controls...primer at least 50bp upstream of the sequence of interest. 5. Use a silica spin column for purification ...

  17. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...deciding which one is right for you, so if you’re interested in learning more about fluorescent proteins, ...

  18. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...can use to express both SpCas9 and your gRNA of interest in a variety of mammalian cell lines. Alternative...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited...
Showing: 321 - 340 of 644 results