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Showing: 321 - 340 of 606 results

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...Pol, the last component, contains the reverse transcriptase and integrase components to convert the cargo...the primary viral polymerase. E4/E1A activate transcription of other relevant viral genes (including the...

  4. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies of their... such as a heterologous envelope or a post-transcriptional regulatory element can be used for either type...

  5. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

  7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...Genome-Wide Control for Large-Scale Functional Screens        ...

  9. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

  10. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    .... Such a system has been previously described for transcriptional activation/repression and genome editing...

  12. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

  13. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  15. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

  16. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

  17. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  18. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

  19. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...
Showing: 321 - 340 of 606 results