We narrowed to 468 results for: SEC

1. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a link to search...

2. Viral Vectors 101: Viral Vector Elements

   Type

   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ...these viral genes is available in the adenovirus section below!   Fig. 2 - Viral vector components...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell line developed...

4. Clear and Accessible Writing

   Type

   Blog Post

   Published

   Feb. 15, 2024, 2:15 p.m.

   ...the first time and learning how to purify AAV.  Second, you’ll identify your purpose in using that image...

5. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ... the following articles are just a small cross-section spanning 20 years of research. DNA Cross-Bridging...

6. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...companies selling antibodies, microscopes, and dissection tools, but there were also people ready to discuss...

7. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ... cellular physiology in your system. Optical sectioning cannot be conducted with luminescence imaging...

8. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...Functional assays for cancer variants To overcome the second major challenge, we developed assays to test large...

9. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...of DNA Dcm methylase adds a methyl group to the second cytosine of CCWGG EcoKI methylase adds a methyl...

10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used. Just ...

11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ... so they won’t work if the fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...tRNA-gRNA constructs or a 1-4 base tail from the second tRNA in the tRNA-gRNA-tRNA constructs. gRNAs with...

13. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...mechanisms – our project wouldn’t be possible. A second technical hurdle is the lack of resources associated...

14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...and software tools in the Materials and methods section of their manuscript to make resources easier to...

15. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...origin of replication (Please note: This first section primarily pertains to ORIs in budding yeast, Saccharomyces...

16. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    Published

    Feb. 4, 2014, 3:50 p.m.

    ...finding someone to give you advice and guidance. Second, I will offer some advice on how to “make the ask...

17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...material downloads. This was particularly helpful for securing funding to support this effort. The future of ...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...

19. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...

20. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...will need to write protocols, SOPs, and methods sections. The next time you write or read one of these,...