We narrowed to 389 results for: control
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TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...
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Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....living cells where methylation of these sites was controlled, the reporter consistently showed significantly... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....conjunction with activator or repressor domains to control gene expression. Base editors can help you make... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....stains as loading controls: An alternative to high-abundance single protein controls in semi-quantitative... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....more sensitive to trimethoprim when compared to control gRNA treated or untreated bacteria. Had the folA... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....action potentials, muscle contractions, and the control of voltage gated ion channels all depend on transmitting... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m.... CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled Libraries Resources on Addgene.org Addgene's... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....opposite task, keeping cells from dividing out of control. One may compare the roles of proto-oncogenes and...same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation... cellular level there is a universal theme - uncontrolled cellular proliferation. We have appreciated ...appreciated for some time the causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m.... enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....of all distributed plasmids as part of quality control, but does not perform functional tests. The newest... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....not that I would know about this… A no template control (NTC) should always be included. While the physical... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ...