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We narrowed to 701 results for: lic

Showing: 321 - 340 of 701 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness...

  2. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References...

  3. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played.... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced... cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one...

  4. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Biology of the NationalAcademy of Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...improve our shipment processes. After reading a publication describing how short term storage on dry ice ...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...exposed to CO2/dry ice. All samples were tested in duplicate. What we found Not all tubes (or rather, not ...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... Glucose Glucose, methanol, glycerol Heterothallic mating Efficient Efficient Inefficient... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...their multiplicity of infection so that a single TF was delivered to each cell, future applications could...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...as they do science outreach and engage with the public, and learn from the process of writing papers and...broader impacts for their work in education and policy and to draw attention and support to work in their...before you complete your work. If you’re giving a public talk, ask a friend who works in a different field...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...their reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...sequences. Fusion PCR was performed to create an amplicon that  was cloned into a plasmid backbone for expression...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  16. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  17. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...transmembrane domain receptors, due to the seven alpha helices that transverse the cellular membrane. The receptor...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  18. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  19. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...demonstrated that pathways projecting to the inferior colliculus (IC) are differentially transduced when using...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  20. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...antibody and content team, we identified what applications should be included and what basic information...through an editing process and final check before publication.   Do research! Nearly everything we write entails...make figures in BioRender or use appropriately licensed and cited figures from papers or image sharing...
Showing: 321 - 340 of 701 results