We narrowed to 336 results for: nam

1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

   Type

   Blog Post

   Published

   Dec. 17, 2020, 1:30 p.m.

   ...genetics.118.301775 Ghanta KS, Doksin GA, Mir A, Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer ...

2. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ...produced from long dsRNA via an RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded...

3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ... inverted in relation to the promoter, hence the name, double-floxed inverted open-reading frame (DIO)...

4. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ...Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise, piggyBac was discovered in the...

5. Quick Guide to All Things Lentivirus

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   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

6. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...fluorophores carry their emission peaks within their names, for example, BV421 and RB545. Fluorophores can ...

7. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   Published

   July 20, 2021, 1:15 p.m.

   ...to line up in single file in a process termed hydrodynamic focusing. This allows each cell to pass through...

8. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   Published

   Oct. 24, 2017, 1:54 p.m.

   ..., USA 39.7392°N 104.9903°W - Erin Golden Hi, my name is Erin and I have spent the last 15 years training...

9. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   Published

   Sept. 10, 2020, 11:45 a.m.

   ... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different...

10. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...DNA scissors: the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins...

11. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...the target. Helpfully, this also includes clone names, which can allow you to search different providers...

12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ... this approach to both the PE2 and PE3 systems, naming the new systems PE4 and PE5, respectively. These...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ... References  1. Sano, Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to...

14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ...carries a spacer targeting the gene of interest and kanamycin resistance E. coli carrying the phage recombineering...

15. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...localization and expression. This base editor was named ABEmax. In 2020, two papers were published describing...

16. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    Published

    Oct. 12, 2023, 1:15 p.m.

    ...Sullivan, T. P., Razmpour, R., Gong, Y., Ng, C., Nammour, J., Maiz, D., Dujardin, S., Ramirez, S. H., Hudry...