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We narrowed to 397 results for: nam

Showing: 1 - 20 of 397 results

  1. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ...meet...  Hmmm, they need a name. And we need your help in finding a good name for Blugene’s virus friend... As with Blugene’s name, we’d like to give their virus friend a gender neutral name. The Addgenies will...  During the month of January, we’ll be taking name suggestions using this form or the hashtag #BlugeneVirusFriend...finalists and we’ll ask you to vote for your favorite name. We hope you submit your best ideas because the ...newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional ...

  2. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This simple piece of ...Database. Step 2: Insert name  Include information about the insert in your plasmid name. This is often a 3-...There are no universal rules for naming plasmids but here are some good guidelines to follow in order..." is often used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid...modification, this should be included in the plasmid name. Mutations are generally listed as the amino acid...either directly before or directly after the insert name.   Example: If the Glutamine at position 295 was...and often how it can be used just by reading the name. For more information about plasmid features and...

  3. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...conference room names. The Addgenie’s favorite conference room naming scheme? Gene names! This is not surprising...surprising for Addgene. But the names we chose were not just any gene names. Now, we have meetings in rooms...Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an organism...conference room names originated from. Let’s take a look at some of the stories behind the names. We also have...communities have a little more leeway in what they can name a gene and many of the interesting ones come from...actually used as a break room for Addgenies, was named after the protein Groucho, a transcriptional co-...the comedian Groucho Marx, so the gene was aptly named groucho (Fisher and Caudy, 1998). Interested in ...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ..., Myers, M. T., Zhou, X., Hou, Z., Lozen, M. L., Nam, K. H., Zhang, Y., & Ke, A. (2024). Exploiting activation... a type? That which we call CRISPR, by any other name would…probably still edit genomes. Understanding...between two types, the Cas effector may have the same name but be completely different! They are merely linked...homology-directed repair. There are 10 subtypes of type V, named A–I and U. V-A is the most popular Cas12 effector...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SOD1 C57D/C146D SOD1 CMV ALS Nam-Chul Ha 191849 pProEx-HTa-SOD1 WT SOD1 ALS Nam-Chul Ha 191850 pProEx-HTa-SOD1...pProEx-HTa-SOD1 C57A/C146A SOD1 ALS Nam-Chul Ha 191851 pProEx-HTa-SOD1 C57D/C146D SOD1 ALS Nam-Chul Ha 191852 pProEx-HTa-SOD1...pProEx-HTa-SOD1 C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy...Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD ...

  7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...common antibody applications. Intro to the Lab Bench Name Description (Link opens in a new window) Link to...laboratory materials on a balance Basic Molecular Biology Name Description (Link opens in a new window) Link to...or cells without a commercial kit Plasmid Cloning Name Description (Link opens in a new window) Link to...consortium for targeting human and mouse genes. Virus Name Description Link to Video General Transfection Introduce... titer of your lentivirus preparation Antibodies Name Description Link to Video Transfection Introduce...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...the sample name, experiment type, supermix type (ddPCR Supermix for Probes), the target names and target...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the sample name, experiment type, supermix type (ddPCR Supermix for Probes), the target names and target...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics with designed ...biosensor by name, target, publication title or keywords (e.g. "RCaMP", "caspase", "Dynamic Visualization...Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20...erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein ...Protein Sensor for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun...fluorescent CEPIA indicators for visualization of Ca(2+) dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835...ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. 2017 Nov 3;358(...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...in DMSO) 25 µg/mL Gentamycin 10 mg/mL 10 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg...

  17. Lentiviral Prep Service

    Type
    Collection
    ..., see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout...or cuts ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI...transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9...-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library...index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 ...Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato...Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP...Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...

  19. Recombinases AAV Preps

    Type
    Collection
    ...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1...phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1...none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-...pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre...

  20. AAV Molecular Tools

    Type
    Collection
    ...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA...purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP...CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype PI 61592 pX600...
Showing: 1 - 20 of 397 results