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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: nin

Showing: 321 - 340 of 816 results

  1. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ... She suggests to pick up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics... the afternoon during the child-rearing years, learning “lots of interesting things from children, their... cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project...

  2. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...scientists. It takes us a decade or more for just the “training” portion of our careers. In that time we may have...organizing mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive...these things and more. It is instructive (and entertaining) to review this list of bad mentor behaviors...openly Gives honest feedback without ever being demeaning Does not micromanage (teaches you to fish, doesn...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...surprisingly simple, requiring only a PCR product containing the modifying sequence flanked by approximately...distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...additional benefit of multiple selection markers for combining modifications within a single strain. In addition...

  4. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...are the three main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector...defined neuronal populations using viral strategies Combining different approaches can increase the specificity...information. To complement these efforts, we’re beginning to share data through our recently launched AAV...

  5. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...conventional microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

  6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...in Drosophila. SPARC was developed by Thomas Clandinin’s lab, and incorporates several common genetic ...both for visualization and for optogenetics. The Clandinin lab used SPARC to image the calcium response in... YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR (2019) SPARC: a method to genetically manipulate...resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... hormone,” it’s also involved in reinforcement learning, decision making, and motor control.  What do... mice Report modulation of DA signaling during learning and reward consumption in live mice Find dLight...Drosophila Detect DA dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish...release in live mice over the course of Pavlovian conditioning Find the GRABDA sensor here! Overall these ...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...keep reading). Corning takes back and recycles a lot of their plastic packaging (from Corning®, Falcon®, ...plastics are ever recycled, so adopting a few meaningful changes in the lab could make a big difference...person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...connections with coworkers and doing something meaningful with my career. I can’t pinpoint exactly when... mine is mainly within content and business.  Joining the Addgene crew!  This pattern was consistent:...bigger projects I was tasked with was creating and sustaining a biweekly report of our social media analytics...free time, she enjoys reading, petting dogs, and winning the office Just Dance competition.    Special ...

  10. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ...away-from-the-bench career option. So if you’re interested in learning more about science communication careers, you’...s paid off. Work is never boring. “I just keep learning on the job. It keeps it interesting.” And while...accomplishment.” He started working on them shortly after joining IDT when his boss was looking for someone to take...never get off of the ground. So limiting that, and defining a couple of key terms is a better approach. Also...

  11. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...make us self-sustaining. With this unique model, we are able to spend resources on maintaining quality and...investors or stock. All of our proceeds go into running the repository and serving scientists better. That... this trust by keeping excellent records and maintaining confidentiality where it is appropriate. This...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...that my piecemeal degree might one day foster a meaningful day job only came into focus when an English ...podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation, graphic design...interested in connecting with the public in a meaningful way, and not solely to ensure a profit. Often...Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro to Our Summer SciComm Series!...

  13. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...the transgene will be lost. Over time, bacteria containing the recombined vector backbone will also outgrow...samples. For a more detailed comparison of these cloning strains, browse the table of common gene mutations...there are both large and small colonies. Bacteria containing the recombined product tend to grow faster, so...N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia...

  14. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all...mentors to listen, guide and support us during training and throughout our careers. An easy way to access...academia and not in academia is run by grant funding. Learning to write grants quickly, efficiently and effectively...Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities L’oreal...

  15. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...when you’re running your next AAV experiment, use these questions as a guide for designing your future...Data Hub to quickly find details they need for planning their next experiments using a specific AAV tool...activate receptors? If you’re in the process of designing your experiment, read our blog post on important...

  16. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists...fused them to sequences called complementarity determining regions (CDRs, or loops) of an antibody that ...bound to HA epitopes for minutes in living cells, meaning that scientists can track the movement and localization... & Givol, D. (1972). Localization of antibody-combining sites within the variable portions of heavy and...

  17. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...instruction. Six of the kits, known collectively as Greening the Classroom, were developed and tested by ABRC...such as instructional videos and datasheets. The remaining kits were donated to ABRC by the Arabidopsis research...has many benefits. Engaging students in active learning through hands-on experiments allows them to develop... out opportunities to engage people in science learning. Whether visiting classrooms, hosting field trips...

  18. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...daily basis, many found these tools inadequate for planning, visualizing, and documenting their procedures...procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer... even if one or two codons are missing at the beginning or end of the feature. With the increasing popularity...people love to use, and likes to start at the beginning, shepherding a new product from the idea stage...

  19. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial ...part of interpersonal communication, which makes honing your ability to tell stories a similarly crucial... with me. First I calmed the situation down by opening a dialogue specifically about the project and contributions...

  20. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...acknowledged perks of scientific and technical training is that these educational paths prepare you for...perspective on other types of science careers as their training progresses.  In From Academic Solos to Industrial...explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless jump into...quickly Jack/Jane of all trades More sources of training and resources within the company Must find own...
Showing: 321 - 340 of 816 results