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...published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory...genes involved in a phenotype of interest. This blog post will discuss differences between these approaches...CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR...

...This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades.... She then moved to France where she worked as a post-doctoral fellow on developmental biology at the ...Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene website Visit our molecular...

...IgG2a? Will my choice affect my experiment? In this post we will review some of the main factors that affect...using polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

...Half (113) expressed in retinal cells four weeks post-injection and 14% had targeted expression in specific...technologies Browse all of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find...mouse retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic...Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez,...

...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...was a PhD student at University College Dublin & a post-doc at Cambridge University where he studied mechanisms...published in a paper, but positive outcomes can include poster presentations, talks about your work, or even the...

...This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First... the Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...2016). We quickly assembled a team of students, postdocs and professors to move the project forward as ...

...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models...

...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...Taylor-Parker contributed to the writing of this post. Additional Resources on the Addgene Blog Visit...

...Tips for Using FRET in Your Experiments Post Read Other Blog Posts about Fluorescent Proteins  ...

...great choice for your next experiment! In this blog post we’ll provide an overview of some CRISPR mammalian...Marcy Patrick contributed to the writing of this post. Additional Resources on the Addgene Blog Check...

...This post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...”, it is certainly the most robust. In this blog post, we will discuss how CRISPR libraries are being ...

...check in regularly to see the newest GIFs we’ve posted, and tag us on social media when you use your favorite...

...Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made...

... your career, whether you’re a graduate student, post-doctoral fellow, lab manager, research technician...

...tips for titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses ...

...improve yield. In addition, as described in this blog post, the team developed a viral genome sequencing strategy...

... For more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial...

... Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at Addgene...them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing...at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is...sub-domains. It has also been used to observe intra- and post-Golgi segregation of cargo during their transport...about these light-switchable TCSs, read our blog post: Synthetic Photobiology: Optogenetics for E. coli...more information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31...using traditional cloning strategies may seem preposterous. Luckily, the Pierre Neveu laboratory designed...

...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies... Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future of Research...chair of the postdoctoral engagement committee and active member of the university's Postdoctoral Association...in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical School working...runs hand in hand with policy. Alexandra is a postdoctoral researcher in the University of Michigan Department...continue the dialogue. Erin Golden is currently a postdoctoral fellow at the University of Colorado Anschutz...

...experiment are known as your "panel". In this blog post, we'll discuss the principles and process of designing...resolve this issue. Make sure to check out our blog post on color compensation to learn more about them. ...bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about the principles behind fluorophores...