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Showing: 321 - 340 of 694 results

  1. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...Half (113) expressed in retinal cells four weeks post-injection and 14% had targeted expression in specific...technologies Browse all of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find...mouse retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic...Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez,...

  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...IgG2a? Will my choice affect my experiment? In this post we will review some of the main factors that affect...using polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...was a PhD student at University College Dublin & a post-doc at Cambridge University where he studied mechanisms...published in a paper, but positive outcomes can include poster presentations, talks about your work, or even the...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First... the Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...2016). We quickly assembled a team of students, postdocs and professors to move the project forward as ...

  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...Taylor-Parker contributed to the writing of this post. Additional Resources on the Addgene Blog Visit...

  8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...great choice for your next experiment! In this blog post we’ll provide an overview of some CRISPR mammalian...Marcy Patrick contributed to the writing of this post. Additional Resources on the Addgene Blog Check...

  9. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...This post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...”, it is certainly the most robust. In this blog post, we will discuss how CRISPR libraries are being ...

  11. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made...

  14. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...tips for titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses ...

  15. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... For more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at Addgene...them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing...at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is...sub-domains. It has also been used to observe intra- and post-Golgi segregation of cargo during their transport...about these light-switchable TCSs, read our blog post: Synthetic Photobiology: Optogenetics for E. coli...more information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31...using traditional cloning strategies may seem preposterous. Luckily, the Pierre Neveu laboratory designed...

  17. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...experiment are known as your "panel". In this blog post, we'll discuss the principles and process of designing...resolve this issue. Make sure to check out our blog post on color compensation to learn more about them. ...bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about the principles behind fluorophores...

  18. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies... Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future of Research...chair of the postdoctoral engagement committee and active member of the university's Postdoctoral Association...in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical School working...runs hand in hand with policy. Alexandra is a postdoctoral researcher in the University of Michigan Department...continue the dialogue. Erin Golden is currently a postdoctoral fellow at the University of Colorado Anschutz...

  19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant...the majority of knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more..., some of the “elegans” methods outlined in this post – particularly the use of co-selection methods –...
Showing: 321 - 340 of 694 results