We narrowed to 373 results for: proc.2

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their... become more actively involved in the learning process. As trainees become more comfortable with the open-ended...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...of students active participants in the research process – so if this sounds like an exciting opportunity...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...plants doesn’t always have to be a time-consuming process. While developing transgenic hairy root lines in... longer, a transient plant leaf transformation process could save the plant biologist some time… months...This method is commonly used in C. roseus, but processes specific to photosynthetic active tissues cannot... In the laboratory we can utilize this natural process by replacing the genes on the T-DNA region with...other aspects of the transient transformation procedure (Mortensen et al., 2019). Our method might provide...

...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

...system (by measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...

...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...!     Figure 1: Flowchart showing the process for using pooled lentiviral libraries. A) Amplification...of your pooled library during the amplification process. It’s far better to verify via NGS now than to ...want to consider spinfection. Spinfection is the process of transducing cells at a concentrated seeding ...

...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct... toxin which can affect a variety of cellular processes and an antitoxin that suppresses the toxin’s activity...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...need fewer resources for the entire translation process. For example, during high growth rate conditions...segregate with the cell pellet during purification procedures. Even if you produce a large amount of protein...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method... products that help to further streamline this process and/or stabilize RNA during tissue collection and...

...1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning Golden Gate cloning...enzyme recognition sites. As a result, the ligation process is close to 100% efficient. Another strength of...

... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...

...protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...because a process is working, doesn’t mean it will always be that way. Things change and processes often ...intentionally engage in a creative problem-solving process?  You may want to start by carving out time to ...holds the dual purpose of giving people time to process the issue properly while also discouraging everyone...is thrown out because they haven’t had time to process on their own. You may also find your meetings run...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl... mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated clones. Forward... introduce undesired mutations through the PCR process, you need a high-fidelity polymerase. There are...fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces an additional restriction...DNA duplex stability from the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986...Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward...target cells Labs tend to follow the same general procedure for lentiviral infections, produce the virus, ...vectors are engineered with non-native envelopes, a process called pseudotyping. Lentiviruses pseudotyped with...Segura, M.M., Kamen, A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy...cellular receptors for vesicular stomatitis virus.” Proceedings of the National Academy of Sciences 110.18 (2013...

...condition.   Fig. 2: Schematic of the goldilocks antibody selection process The exposure time ...

.... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...

...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...Fluorescent RNA aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged...for qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences... Aptamer Fluorophores describes the enrichment process used to evaluate oligonucleotides for binding affinity...affinity to a specific target, and the molecular processes that make aptamer biology a powerful tool. In ...

...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...protein. At some point during this interrogation process, they will almost invariably say something like...

... already have an end product in mind, steps 1 and 2 will generally be straightforward. Likewise for step...that need to be considered during the planning process. For CRISPR experiments, the following framework...