... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...

...protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...because a process is working, doesn’t mean it will always be that way. Things change and processes often ...intentionally engage in a creative problem-solving process?  You may want to start by carving out time to ...holds the dual purpose of giving people time to process the issue properly while also discouraging everyone...is thrown out because they haven’t had time to process on their own. You may also find your meetings run...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl... mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated clones. Forward... introduce undesired mutations through the PCR process, you need a high-fidelity polymerase. There are...fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces an additional restriction...DNA duplex stability from the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986...Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward...target cells Labs tend to follow the same general procedure for lentiviral infections, produce the virus, ...vectors are engineered with non-native envelopes, a process called pseudotyping. Lentiviruses pseudotyped with...Segura, M.M., Kamen, A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy...cellular receptors for vesicular stomatitis virus.” Proceedings of the National Academy of Sciences 110.18 (2013...

...condition.   Fig. 2: Schematic of the goldilocks antibody selection process The exposure time ...

.... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...

...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...Fluorescent RNA aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged...for qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences... Aptamer Fluorophores describes the enrichment process used to evaluate oligonucleotides for binding affinity...affinity to a specific target, and the molecular processes that make aptamer biology a powerful tool. In ...

...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...protein. At some point during this interrogation process, they will almost invariably say something like...

... already have an end product in mind, steps 1 and 2 will generally be straightforward. Likewise for step...that need to be considered during the planning process. For CRISPR experiments, the following framework...

...PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...

...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...

... Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed...no PCR, no gel purification; the whole cloning process is so simplistic once you have your parts. That...

...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...actually share many biological properties and processes with our own cells. Like human cells, yeast DNA...proteins and tissues to better assess developmental processes. For example, the Stainier lab studied the proliferation...making it ideal to study basic molecular biology processes. C. elegans are also quite amenable to genetic...investigate an array of biological systems and processes including the nervous system, development, cell...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...

...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery... to genome editing of mouse zygotes, through a process known as CRISPR-EZ (CRISPR RNP Electroporation ...

...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...consequence of the undermining of the scientific process and the marginalization of science, particularly...

... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...with an ancestry-based bias.   The correction process does not stop there. Currently, they are working...

...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...

... Methods 2009; 6, 343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey... menu of common cloning options in the deposit process in 2014 because of its gain in popularity. Gibson... the Miller Lab Protocol). The Gibson assembly process can be used to assemble up to 6 fragments in one...time commitment. Another advantage is that this process makes it easy to generate wild type and mutant ...drawback to the Gibson assembly technique is that the process works best with fragments over 200 nucleotides....